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Updated: Jul 6, 2025

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
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Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Published on: July 1, 2022

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在倾斜的冷EM数据收集过程中克服分辨率减弱

Sriram Aiyer1, Philip R Baldwin1,2, Shi Min Tan3

  • 1Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.

Nature communications
|January 9, 2024
PubMed
概括

电子显微镜 (cryo-EM) 研究人员现在可以通过阶段倾斜来克服首选样本定向问题. 新的工作流程将分辨率损失最小化,使高分辨率结构确定和分析新型分子相互作用成为可能.

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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