模拟的微重力促进了沙门氏菌在生菜中的口腔进入,并抑制了生物控制剂
Noah Totsline1,2, Kalmia E Kniel3, Chandran Sabagyanam4
1Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19713, USA. noaht@udel.edu.
Scientific reports
|January 9, 2024
概括
太空飞行条件可能会增加食物传播病原体的风险. 微重力影响了生菜口腔,允许更多的Salmonella enterica Typhimurium进入并减少有益的细菌.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 空间生物学 空间生物学
背景情况:
- 在太空飞行中种植作物对于宇航员健康至关重要,他们面临微重力和氧化应激等挑战.
- 食品传播的病原体,如Salmonella enterica,通过绿叶蔬菜构成风险,已知的病原体在国际空间站上持续存在.
- 植物,病原体和微重力之间的相互作用,特别是口腔动力学,仍然不太了解.
研究的目的:
- 为了研究模拟微重力对生菜口腔动态和沙门氏菌进入Typhimurium的影响.
- 评估Bacillus subtilis UD1022作为生物控制剂在模拟微重力条件下对S. enterica Typhimurium的疗效.
主要方法:
- 菜 (Lactuca sativa L.) 植物被注射了S. enterica Typhimurium,并使用2Dclinostat (2和4RPM) 进行模拟微重力.
- 使用共聚焦显微镜测量了口腔孔径宽度和病原体侵入深度.
- 使用连续稀释涂层量化细菌种群,并测试了Bacillus subtilis UD1022的疗效.
主要成果:
- 模拟的微重力显著改变了口腔孔径宽度,S. enterica Typhimurium在旋转下增加了孔径大小.
- 观察到更大的S. enterica Typhimurium入深度和植物种群在旋转 (4 RPM) 下的生菜中.
- 以每分钟4转的旋转抑制了Bacillus subtilis UD1022限制口腔开口的能力,并降低了其生物控制功效.
结论:
- 微重力条件可能会通过改变植物病原体相互作用,加剧基于太空的农业中的食品安全风险.
- 模拟的微重力显著损害了促进植物生长的草原细菌 (PGPR) 控制病原体进入的有效性.
- 这些发现突出了太空飞行食品安全的独特挑战,以及对微重力的影响进行进一步研究的需要.
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