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Juan Long¹, Yang Zeng¹, Fei Liang¹

¹Department of Immunology and National Center for Biomedicine Analysis, Senior Department of Hematology, Fifth Medical Center of Chinese PLA General Hospital, No.8, Dongda Ave, Fengtai District, Beijing, 100071, China.

|January 10, 2024

概括

减弱型沙门氏菌型菌SL7207被设计为口服PCDNA-CCOL2A1DNA疫苗. 这种新的口服DNA疫苗表现出稳定性和体内表达,为风湿性关节炎免疫耐受性提供了有前途的方法.

科学领域:

生物技术是生物技术.
疫苗学疫苗学疫苗学
免疫学免疫学免疫学

背景情况:

口服DNA疫苗代表了预防和治疗疾病的创新方法.
类风湿性关节炎 (RA) 是一种慢性炎症疾病,影响关节.
诱导免疫耐受性是管理RA等自身免疫性疾病的关键策略.

研究的目的:

利用减弱细菌开发一种稳定可控的口服DNA疫苗制剂.
构建和验证由沙门氏菌型流体SL7207.7.传递的pcDNA-CCOL2A1 DNA疫苗.
评估工程口服DNA疫苗的体外和体内稳定性和表达.

主要方法:

设计的PCDNA-CCOL2A1等离子体DNA疫苗.
通过电穿孔转化减弱的沙门氏菌Typhimurium SL7207.通过电穿孔.
通过耐性板测试证实了成功的转化.
通过PCR,测序和小鼠体内研究验证了菌株鉴定,稳定性和基因表达.

主要成果:

成功构建了SL7207/pcDNA-CCOL2A1口服DNA疫苗菌株.
证实了转化菌株在4°C下100天的稳定性.
在口服后检测到CCOL2A1基因表达在小鼠的内皮.
在潜伏后6-8小时内,证明了稳定的细菌度 (10^9 CFU/mL).

结论:

改造的SL7207/pcDNA-CCOL2A1菌株是口服DNA疫苗接种的可行载体.
这种口服DNA疫苗表现出良好的稳定性和体内表达能力.
开发的疫苗为口服免疫提供可控制的剂量,可能诱导对类风湿性关节炎等疾病的免疫耐受性.

关键词:

减弱型沙门氏菌型菌SL7207 SL7207 这种疫苗是DNA疫苗.口服免疫口服免疫的方法稳定的稳定性稳定的稳定性转化菌株SL7207/pcDNA-CCOL2A1 的转化菌株

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