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使用xMAP技术检测真菌毒素的免疫试验的开发及其在黑茶样本中的评估

Ishrat Jahan Saifi^1,2, Manoj Kumar¹, Kamlesh Maurya¹

¹Food Toxicology Laboratory, Food, Drug, and Chemical Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, Uttar Pradesh 226001 India.

Journal of food science and technology

|January 10, 2024

概括

一种新的Luminex悬浮剂测定方法可以同时检测黑茶中的阿弗拉托辛B1 (AFB1) 和奥克拉托辛A (OTA). 这种快速,高通量方法为食品中的真菌毒素监测提供了灵敏而准确的替代方案.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

菌毒素,如阿弗拉托克辛B1 (AFB1) 和奥克拉托克辛A (OTA),是真菌的二次代谢产物,由于其毒性,它们对公众健康构成重大风险.
常规的真菌毒素检测方法 (HPLC,LCMS,ELISA) 往往是昂贵和劳动密集型的,限制了它们在大型食品监测中的应用.
对于食品商品中准确的真菌毒素分析而言,迫切需要强大,快速响应和高吞吐量技术.

研究的目的:

开发一种使用多分析剂分析 (xMAP) 技术的多重化Luminex悬浮试验.
为了在黑茶样本中同时检测AFB1和OTA.
提供一种敏感,准确和快速的查方法,以检测食品中的真菌毒素污染.

主要方法:

开发基于xMAP技术的多重混合Luminex悬浮试验.
在黑茶矩阵中同时检测AFB1和OTA.
测定灵敏度和特异性的验证,包括评估与其他真菌毒素的交叉活性.

主要成果:

开发的Luminex试验实现了AFB1的0.06 ng/ml和OTA的0.49 ng/ml的检测极限.
该试验没有与其他测试的真菌毒素产生交叉反应,证实了其特异性.
该方法被证明适合在同一样本中同时检测AFB1和OTA.

结论:

开发的Luminex悬浮试验是一种敏感,准确和快速的用于检测真菌毒素的工具.
这种测试非常适合在食品商品如黑茶中对多种真菌毒素,特别是AFB1和OTA进行高通量查.
该技术为确保食品安全和监管合规性提供了传统方法的可行替代方案.

关键词:

非洲毒素B1是什么?黑茶是什么黑茶是什么黑茶是什么菌类毒素 (Mycotoxins) 是一种毒素.奥克拉托克辛A是一种采用xMAP技术,可以实现xMAP.

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