相关实验视频

Updated: Jun 17, 2026

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

31.5K

使用DIA SILAC改进了RNA相互作用蛋白质的检测和一致性

Thomas C J Tan1, Christos Spanos1, David Tollervey1

  • 1Wellcome Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh. Edinburgh EH9 3BF, Scotland, UK.

Nucleic acids research
|January 10, 2024
PubMed
概括

这项研究引入了一种更敏感的方法,用于识别使用数据独立获取 (DIA) 的RNA结合蛋白,并使用SILAC标签. 这种方法提高了蛋白质检测,并降低了与传统方法相比的成本.

更多相关视频

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

9.5K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K

相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...