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在老鼠血中同时估计拉洛西芬,克拉德林的LC-MS/MS方法:在药理动力学研究中的应用

Divya Chauhan^1,2, Shailesh Dadge^1,2, Pavan K Yadav^1,2

¹Division of Pharmaceutics & Pharmacokinetics, Central Drug Research Institute, Lucknow, Uttar Pradesh, 226031, India.

|January 10, 2024

概括

一种新的LC-MS/MS方法可以同时准确量化raloxifene (RL) 和cladrin (CL). 这种经过验证的试验为开发结合性骨质疏松症治疗提供了关键的药理动力学数据.

科学领域:

分析化学分析化学
药理动力学药理动力学
生物医学科学生物医学科学

背景情况:

拉洛西芬 (RL) 和克拉德林 (CL) 是潜在的治疗药物.
同时量化RL和CL对于药理动力学研究至关重要.
骨质疏松症的治疗可能受益于联合药物输送.

研究的目的:

开发和验证一种新的LC-MS/MS方法,用于同时量化RL和CL.
在生物矩阵中确定RL和CL的药理动力学概况.
评估结合RL和CL治疗骨质疏松症的可行性.

主要方法:

液态染色学-并联质谱学 (LC-MS/MS) 用于药物分析.
一个RP-18 Xbridge Shield柱被使用了乙尼和酸移动阶段.
该方法在雌性Sprague-Dawley大鼠的生物矩阵中得到了验证.

主要成果:

通过LC-MS/MS方法,在1-600 ng/ml度范围内证明了线性.
对于RL和CL,量化的下限为1 ng/ml.
确定RL和CL的药理动力学参数 (Cmax,Tmax).

结论:

经过验证的LC-MS/MS方法适用于同时量化RL和CL.
生成的药理动力学数据将有助于开发组合RL和CL配方.
这项研究支持未来的骨质疏松症治疗的联合药物输送策略.

关键词:

在 LCMS/MS 中使用.生物分析方法验证共同估计的估计.药物动力学药物动力学拉洛西芬和克拉德林的使用方法同时识别的同时识别.

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