相关实验视频

Updated: Jul 6, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

开发,识别和利用耐条纹生的小麦四分体Thinopyrum elongatum 4EL转位线路

Biran Gong^1,2, Linfeng Chen^1,2, Hao Zhang^1,2

¹State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|January 10, 2024

概括

在Thinopyrum elongatum中发现了一种新的条纹抗基因Yr4EL,并转移到小麦中. 这种基因提供了高耐药性,可以用于未来的小麦育种计划,以改善作物保护.

科学领域:

植物遗传学和育种.
提高作物质量提高作物质量
在小麦的疾病耐药性.

背景情况:

薄皮 (Thinopyrum elongatum) 是一种有价值的遗传资源,可以增强小麦对疾病的抵抗力.
在T.的染色体4E的TH. 长类动物携带条纹生抗性基因.
之前的研究发现了染色体4E上的条纹生抗性.

研究的目的:

来识别和转移一个条纹抗基因从四体Th. 长植物变成普通小麦.
开发转位线,以优化小麦育种中的基因效用.
为了将抗性基因定位到特定的染色体臂.

主要方法:

使用60Co-γ辐射诱导染色体结构变化.
开发单体置换和转位线的发展.
使用GISH,FISH,FISH-painting和小麦55K SNP阵列分析进行确认.
开发SSR标记器用于标记器辅助选择 (MAS).

主要成果:

已经确定了四个含有4EL染色体臂的转位线 (T4DS·4EL,T5AL·4EL,T3BL·4EL,T5DS·4EL).
在这些转位线上观察到高条纹抗性.
一个条纹抗基因,Yr4EL,被定位在4EL染色体臂.
开发了32个特定于4EL和Yr4EL共分离的SSR标志物.
4DS·4EL系列表现出改善的农学特征.
Yr4EL已成功转移到三种小麦品种 (SM482,CM42,SM51) 中,从而赋予了条纹抗性.

结论:

基因Yr4EL来自四平状体Th的基因. 长种在普通小麦中赋予了高条纹的抗性.
转位线和已开发的标记物有助于在小麦品种计划中应用Yr4EL.
Yr4EL 是一种有价值的遗传资源,可用于开发抗条纹生的小麦品种.

更多相关视频

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Author Spotlight: Integrating Biochemical Functions of β-Glucanases and Peroxidase Enzymes in Wheat-RWA Interaction

Published on: July 26, 2024

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

相关概念视频

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...