相关实验视频

Updated: Aug 10, 2026

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Published on: November 3, 2011

珀莱肯 (HSPG2) 促进人类心肌细胞的结构,收缩和代谢发育

Benjamin B Johnson¹, Marie-Victoire Cosson², Lorenza I Tsansizi²

¹Centre for Metabolic Health, Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich NR4 7UQ, UK.

|January 10, 2024

概括

珀莱肯 (HSPG2) 促进心肌细胞成熟,稳定心脏.

科学领域:

心血管生物学心血管生物学
细胞外矩阵生物学细胞外矩阵生物学
干细胞生物学干细胞生物学

背景情况:

珀莱肯 (HSPG2) 是一种关键的细胞外基质 (ECM) 成分,对组织发育至关重要.
它在心肌细胞成熟和心脏功能中的特定作用仍然在很大程度上未被定义.

研究的目的:

研究珀莱干 (HSPG2) 在心肌细胞成熟和心脏组织发育中的作用.
阐明人体多能干细胞衍生心肌细胞 (hPSC-CMs) 中珀莱肯缺乏的功能后果.

主要方法:

在体内和体外心肌细胞发育过程中,分析了perlecan表达的分析.
在hPSC-CM中区分perlecan-haploinsufficient (HSPG2+/-) 的hPSC.
评估hPSC-CMs中的结构,收缩,代谢和基因表达特征.
对缺乏珀莱坎-哈普洛因的工程心脏组织 (EHTs) 的评估.
对培养在珀莱干基板上的hPSC-CMs的研究.

主要成果:

随着心肌细胞的成熟,珀莱肯的表达增加.
在hPSC-CMs中的珀莱肯缺乏导致结构性,收缩性,代谢性和ECM基因失调,促进不成熟的特征.
具有哈普洛因不足的EHT表现出减少的厚度和力生成.
珀莱肯促进细胞扩大和核形成,表明过度缩的生长,与agrin的作用形成鲜明对比.

结论:

珀莱肯 (HSPG2) 对于促进心肌细胞成熟和稳定心脏组织至关重要.
珀莱干信号传递,可能是通过双甘复合体,抵消增生和增殖,有利于成熟.
准依赖珀莱肯的途径可能通过逆转不适应的细胞变化,为心力衰竭提供治疗策略.

关键词:

CP: 细胞生物学细胞生物学 CP: 发育生物学亚格林亚格林是一家农业公司.心脏成熟的过程这是心肌细胞 (cardiomyocytes).细胞外矩阵是细胞外矩阵.肝硫酸盐蛋白质糖是一种蛋白质糖.人类多能干细胞干细胞过度缩的过度缩是一个问题.多核化的多核化.佩尔莱卡纳 (Perlecan) 是一个巨人.珀莱肯突变变异的突变

更多相关视频

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Published on: June 19, 2018

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Published on: March 24, 2023

相关概念视频

Proteoglycans

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...

Healing I: Introduction

Healing I: Introduction

Healing is the physiological process by which the body restores the integrity and function of damaged tissues following injury. It involves a coordinated interplay of cellular proliferation, extracellular matrix remodeling, and growth factor signaling. The extent and nature of the tissue damage determine whether healing occurs by resolution, regeneration, or replacement.ResolutionResolution represents the most complete form of healing, occurring when the injury is minimal and tissue...