纤维细胞诱导的乳腺上皮分支取决于纤维细胞收缩性
Jakub Sumbal1,2,3, Silvia Fre2, Zuzana Sumbalova Koledova1
1Masaryk University, Faculty of Medicine, Department of Histology and Embryology, Brno, Czech Republic.
PLoS biology
|January 10, 2024
概括
纤维细胞收缩性对于乳腺上皮质分支形态发生是必不可少的. 这项研究揭示了纤维细胞的物理相互作用和机械力量如何驱动上皮细胞的发育,影响器官形成.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 组织工程是组织工程.
背景情况:
- 表皮分支形态发生对于器官发育至关重要.
- 流体微环境,特别是纤维细胞在指导这一过程中的作用尚未完全理解.
- 了解这些相互作用是理解器官形成和相关疾病的关键.
研究的目的:
- 为了研究纤维细胞收缩在乳腺上皮质分支形态的作用.
- 阐明纤维细胞影响表皮细胞发育的分子机制.
- 探索在发育和疾病上下文中的潜在影响.
主要方法:
- 纤维细胞与乳腺上皮有机体/球体的共同培养系统.
- 时间间隔成像用于观察动态细胞相互作用和分支.
- 药理上抑制ROCK和非肌肉肌肉蛋白II.
- 基因操纵 (Myh9的纤维细胞特异性淘汰).
- 对Yes相关蛋白 (YAP) 活动的分析.
- 在小鼠乳腺中的体内研究.
主要成果:
- 乳腺上皮质分支需要身体接触和纤维细胞收缩性.
- 抑制ROCK,非肌肉肌肉蛋白II或Myh9可以消除纤维细胞诱导的分支.
- 表皮增殖和YAP活动模式取决于纤维细胞收缩性.
- 收缩性纤维细胞被确定在体内周围的终端末端芽.
结论:
- 纤维细胞收缩性是一种新的和关键的 stromal 因素驱动乳腺上皮形态发生.
- 这种机械信号通路对于正常的上皮细胞发育至关重要.
- 这些发现提供了关于机械活性纤维细胞在发育与瘤发生过程中的不同作用的见解.
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