使用DNA-PAINT在纳米尺度上比较植物性和胞性Bacillus subtilis之间的分裂组织
Kimberly Cramer1,2, Susanne C M Reinhardt1,2, Alexander Auer1,2
1Faculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
Science advances
|January 10, 2024
概括
细菌的分裂在植物和分泌模式之间有所不同. 超分辨率显微镜揭示了独特的蛋白质组合和放置,影响纳米级的细胞分裂.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 显微镜的使用方法
背景情况:
- 形成子的细菌表现出两种不同的细胞分裂过程:植物分裂和化.
- 划分机械的关键部件Z环的位置因划分模式而异.
- 了解这些模式之间的分工机械组装中的纳米尺度差异至关重要,但具有挑战性.
研究的目的:
- 为了比较关键分裂蛋白 (SepF,Zapa,DIVA,FtsZ) 在植物性和分泌细菌细胞中的3D组装和分布.
- 为了研究植物性和分泌细菌之间的细胞分裂机制中的纳米尺度差异.
- 提出一个模型,用于分工机械的安置和调节.
主要方法:
- 使用DNA-PAINT超分辨率显微镜实现纳米级分辨率.
- 分析了SepF,Zapa,DivIVA和FtsZ蛋白质的3D组装和空间分布模式.
- 植物性细胞中的蛋白质组织与运动细胞中的蛋白质组织的比较.
主要成果:
- ZapA和SepF在植物性和分泌细胞中与Z环类似地局部化.
- 在DIVIVA组件中,植物细胞和胞细胞之间呈现出明显的模式.
- 不管分裂模式如何,SepF形成了~50纳米的弧形.
- 在Z环和早期分体蛋白的对称/不对称位置上发现了差异.
结论:
- 细菌细胞分裂机械表现出不同的纳米级组织在植物和子模式之间.
- Z环和早期分体蛋白的位置决定了对称或不对称的分裂.
- 隔膜厚度的调节在植物分裂和分泌之间有所不同.
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