多细胞球体的生物组装,以模仿复杂的组织结构,使用表面修饰的磁化纳米纤维
Hayeon Byun1,2, Sangmin Lee1,2, Heungsoo Shin1,2,3
1Department of Bioengineering, Hanyang University, 222 Wangsimri-ro, Seongdong-gu, Seoul 04763, Republic of Korea.
Biofabrication
|January 10, 2024
概括
研究人员开发了磁纳米纤维,以精确控制器官建模的微组织结构. 这种生物制造方法使得无脚手架的功能,多域微组织的创建,具有增强的抗氧化特性和磁性响应.
科学领域:
- 生物制造和组织工程
- 材料科学在医学中的科学.
- 再生医学是一种再生医学.
背景情况:
- 在生物制造中,创建没有支架的复杂器官模型是一个重大挑战.
- 现有的方法难以实现对微组织结构和功能的精确控制.
- 对于更真实的有机体开发,需要无脚手架的方法.
研究的目的:
- 引入一种使用表面可修改磁纳米纤维的无脚手架微组织构造的新方法.
- 为了证明精确控制球形功能和几何,用于创建多域微组织.
- 探索这种方法在器官建模和再生医学中的潜力.
主要方法:
- 在poly-L-乳酸中的磁纳米粒子的电,以创建磁纳米纤维.
- 用聚多巴胺 (PD) 涂层纳米纤维来增强生物功能,包括反应性氧物种 (ROS) 清理.
- 将PD涂层磁纤维 (PMF) 纳入人类皮肤纤维细胞球体,并使用磁力操纵组装结构.
主要成果:
- PMF向球体传递了磁性反应,使其能够组装成各种3D结构 (线性,三角形,方形).
- PMFs上调了抗氧化剂基因表达 (超氧化脱氧酶-1,谷氨过氧化酶-1),赋予了ROS对球体的耐药性.
- 组装的含有纤维细胞和内皮细胞的微组织在磁性操纵下模仿了血管结构.
结论:
- 表面可修改的磁纳米纤维可以精确控制微组织的形成和功能.
- 这种无支架的生物制造技术有助于创建复杂的多域微组织.
- 该方法对推进器官建模和再生医学应用具有重大前景.
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