Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Immune - cell death index in hepatocellular carcinoma: a multi-omics and machine learning study for prognosis and immunotherapy prediction.

Frontiers in immunology·2026
Same author

Covalent Ligand-AChE Adduct is a Bidirectional Indicator for Either Ligand or AChE: Pesticides as the Case.

Analytical chemistry·2026
Same author

Mild hyperbaric oxygen enhanced the regeneration of the tibialis anterior muscle after the cardiotoxin-induced muscle injury.

Biochemistry and biophysics reports·2026
Same author

Whole-transcriptome insights into light-responsive non-coding RNA networks regulating circadian clock and DNA repair in zebrafish.

Functional & integrative genomics·2026
Same author

Disparities in obesity-related cardiovascular-induced mortality trends in the U.S. of America over two decades.

Frontiers in cardiovascular medicine·2026
Same author

Developmental exposure to environmental levels of sulfamethoxazole (SMX) leads to hyperactivity in zebrafish larvae by inducing oxidative stress and calcium dyshomeostasis.

Journal of hazardous materials·2026

相关实验视频

Updated: Jul 6, 2025

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
08:02

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Published on: April 25, 2016

9.7K

综合后获取数据处理策略,用于对的胆囊快速表征类固醇硫酸盐.

Wei Chen1, Wenhui Zhao1, Lijuan Wu1

  • 1Modern Research Center for Traditional Chinese Medicine, Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.

Journal of pharmaceutical and biomedical analysis
|January 10, 2024
PubMed
概括

这项研究介绍了一种使用液态染色体质谱法 (LC-MS) 快速化学过中国药物材料的综合策略. 该方法有效地分析了青胆囊中的类固醇硫酸盐,有助于对复杂的自然产品进行表征.

关键词:
(34) S 是同位素分子离子.比利科尔硫酸盐是什么意思获取后数据处理策略 获取后数据处理策略类固醇硫酸盐是什么 类固醇硫酸盐青的胆囊 - - 青的胆囊

更多相关视频

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

9.6K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K

相关实验视频

Last Updated: Jul 6, 2025

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
08:02

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Published on: April 25, 2016

9.7K
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

9.6K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K

科学领域:

  • 分析化学 分析化学
  • 自然产品化学 自然产品化学
  • 药理学是指药理学,即药理学是指药理学.

背景情况:

  • 液体染色学-质谱学 (LC-MS) 对于鉴定中国药物材料 (CMM) 是至关重要的,因为它拥有丰富的MS/MS光谱数据.
  • 从LC-MS处理大数据集以识别特定化合物具有挑战性和耗时,阻碍了CMM的高效分析.
  • 类固醇硫酸盐,包括bufogenin,胆酸和胆醇硫酸盐,是CMM中发现的重要生物活性化合物,如胆囊.

研究的目的:

  • 开发和验证一个集成的数据过策略,以快速,以同类为重点的化学特征CMMs使用LC-MS.
  • 将这种策略应用于胆囊 (Chandan) 中类固醇硫酸盐的深入分析.
  • 证明该策略在复杂的自然产品矩阵中对子代谢体表征的实用性.

主要方法:

  • 开发了一种综合策略,结合了分子量印记 (MWI),诊断断片离子过 (DFIF),中性损失过 (NLF) 和同位素模式过 (IPF).
  • 在实验室代谢中使用复合人体SULT2A1生成的布夫原硫酸盐来确定MS/MS光谱行为.
  • 来自胆囊的LC-MS/MS数据使用MWI,IPF,DFIF (准SO3·和HSO4) 和NLF (准SO3) 进行过,用于硫酸盐的识别.

主要成果:

  • 综合策略成功过并识别了胆囊中的71种化合物,包括7种布夫原硫酸盐,17种胆酸硫酸盐和13种胆醇硫酸盐.
  • 在检测到的化合物中,有39个化合物根据实证质量碎片化规则和光谱数据被赋予了合理的身份.
  • 该方法使得的胆囊中类固醇硫酸盐的综合概况成为可能,展示了其对复杂天然产品分析的有效性.

结论:

  • 拟议的综合数据过策略显著加快了使用LC-MS/MS的CMM的同类聚焦化学表征.
  • 这种方法为在复杂的天然产品样本中深入分析特定化合物类 (如类固醇硫酸盐) 提供了有价值的工具.
  • 该策略为CMM和潜在的其他复杂生物矩阵中的亚代谢体表征提供了一个有意义的选择.