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Rongshuai Yuan¹, Bolin Cai¹, Manting Ma¹

¹State Key Laboratory of Livestock and Poultry Breeding, Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Research Center for Livestock Breeding, Guangzhou, China.

Poultry science

|January 10, 2024

概括

在LncEDCH1中,单核酸多态 (SNP) 与的肌肉和皮肤特征有关. 微RNA-196-2-3p调节LncEDCH1,影响肌肉发育,为家禽养殖提供标记物.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
禽畜科学禽畜科学禽畜科学

背景情况:

单核酸多态 (SNP) 是理解种群多样性的关键遗传标记.
LncEDCH1以前与的肌肉发育有关.
在家禽繁殖计划中,SNP分析对于加速特征选择至关重要.

研究的目的:

研究LncEDCH1在肉肌肉发育中的特定机制.
在LncEDCH1中识别与经济重要特征相关的SNP.
阐明 miR-196-2-3p 和 LncEDCH1.1 之间的监管关系.

主要方法:

在LncEDCH1.1.上进行了SNP检测,基因型定型,链接不平衡和单元型分析.
使用了双化酶记者测定,miRNA拉下,EDU测定,免疫光和qPCR.
对已识别的SNP和特征进行了统计关联分析.

主要成果:

在LncEDCH1中确定了16个SNP,其中几个与乳腺肌肉重量,腿部重量和皮肤黄色有显著的关联.
两个哈普洛型块与各种皮肤黄色和尸体重量特征有显著的关联.
证实miR-196-2-3p通过与特定的SNP位点 (g.1703613T>C) 结合直接抑制LncEDCH1表达,间接调节肌肉发育.

结论:

在LncEDCH1中识别的SNP可以作为养的有价值的分子标记.
该miR-196-2-3p/LncEDCH1调节途径提供了关于肌肉发育机制的见解.
这项研究提高了对家禽遗传调节的理解.

关键词:

在LncEDCH1中.尸体的特征是尸体的特征.这些都是肉肉.在 miR-196-2-3p 中.单个核酸的多态性.

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