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BeWo外聚物被丰富为生物活性细胞外胎盘特异的C19MC小RNAs

Syunya Noguchi¹, Shohei Tozawa², Takanobu Sakurai¹

¹Department of Molecular Medicine and Anatomy, Nippon Medical School, Tokyo 113-8602, Japan.

Journal of reproductive immunology

|January 10, 2024

概括

胎盘 trofhoblast 细胞释放外体,新的纳米粒子与外体相区别. 这些外体携带更多的胎盘特异性microRNAs (miRNAs),并有效地传递它们用于细胞通信.

科学领域:

细胞生物学细胞生物学
细胞外囊泡细胞外囊泡
分子生物学分子生物学

背景情况:

细胞外囊泡 (EVs),包括外体,是已知的细胞外微RNA (miRNAs) 的载体.
异构体是一种非膀外细胞纳米粒子 (NVEP),代表了一种新型的细胞外载荷载体.
胎盘 trofhoblast-derived 异构体的特征和功能仍然在很大程度上未被探索.

研究的目的:

为了表征热囊细胞衍生的外聚物.
为了研究由这些外构体携带的胎盘特异性miRNAs介导的细胞间通信.
在货物和交付效率方面,将外相与外相相比较.

主要方法:

使用了采用BeWo热囊细胞和Jurkat T细胞的体外模型系统.
使用纳米粒子分析 (大小,形态) 和标记表达 (AGO2,CD63,CD9,CD81,FLOT1,TSG101) 进行了BeWo外体和外体的特征.
量化了胎盘特异性的miRNA水平,并评估了受体细胞中的miRNA输送和基因调控 (PRKG1).

主要成果:

贝沃外体表现出明显的"状"纳米粒子结构 (∼40 nm),与杯状外体 (∼90-110 nm) 不同.
BeWo细胞分泌的外体比外体更多,外体对AGO2呈阳性,对外体标记物呈阴性.
异构体显示出明显更高的胎盘特异性miRNAs水平,并与外构体相比,向Jurkat细胞输送更多miR-517a-3p,导致PRKG1表达的抑制.

结论:

热囊细胞衍生的外体是独特的NVEP,富含生物活性,胎盘特异性的miRNAs.
这些外体有效地传递miRNA用于细胞间通信,影响受体细胞中的基因表达.
这些发现突出了外体在胎盘生物学中的重要作用,挑战了外体在携带某些细胞外miRNA货物的独特作用.

关键词:

异构体的外构体是一种外构体.细胞外囊泡细胞外囊泡这是一个微型RNA.非气囊外细胞纳米粒子非气囊外细胞纳米粒子.热细胞热细胞

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