人类介质体细胞粘附和扩张在用富含氧气和的等离子体聚合物修改的聚合物表面上
Balaji Ramachandran1, Gad Sabbatier1, Olivia M Bowden1
1Department of Chemical Engineering, McGill University, Montréal, Canada.
Colloids and surfaces. B, Biointerfaces
|January 10, 2024
概括
新的等离子聚合物涂层增强了化乙烯 (FEP) 容器上的细胞粘附. 这些经过修改的FEP表面支持人间介质层细胞 (hMSC) 的扩张,这证明了细胞治疗生物制造的前景.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 表面化学 表面化学
背景情况:
- 化乙烯 (FEP) 为细胞生物制造提供了可取的特性,如化学惰性和高氧透性.
- 然而,FEP固有的表面特性阻碍了依赖结的细胞粘附和存活,限制了其应用.
- 现有的局限性需要表面修饰策略来提高FEP的生物相容性,以治疗细胞扩张.
研究的目的:
- 开发用于修改FEP表面的新型等离子体聚合物涂层.
- 在涂层的FEP容器上增强人类介质干细胞 (hMSCs) 的粘附和扩张.
- 与标准组织培养聚烯相比,评估修改FEP表面的性能.
主要方法:
- 在FEP基板上制造可调节的富含氧或富含的表面化学物质的等离子聚合物涂层.
- 在蒸汽杀菌后评估涂层稳定性和性能.
- 在各种条件下,包括蛋白质的存在和缺乏,对涂层FEP表面的hMSC粘附和扩张的评估.
- 对细胞粘附机制的分析,区分因特林介导和物理化学结.
主要成果:
- 等离子聚合物涂层成功修改了FEP表面,实现了富含氧气或富含的化学物质.
- 蒸汽灭菌保持了涂层的湿透性和表面化学特征.
- 富含的涂层显示了更高的白蛋白吸附,与增强的hMSC扩张相关.
- 与未经处理的FEP相比,富含氧气和的涂层显著改善了hMSC的粘附和扩张.
- 富含的涂层FEP实现了hMSC产量,与标准组织培养处理的聚烯相比.
结论:
- 等离子聚合物涂层有效地提高了hMSC在FEP表面的粘附和扩张.
- 修改后的FEP表面,特别是富含的表面,为细胞治疗应用提供了可行的聚烯替代品.
- 这项技术在扩大基于FEP的培养容器在生物制造中的实用性方面具有重大前景.
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