Sarcocystis spp. 的形态和分子表征. 在来自阿根廷的猪 (Sus scrofa domestica) 中
Elisa Helman1, Andrea Dellarupe1, Kevin Denis Steffen1
1Argentinean National Council of Scientific and Technological Research (CONICET), Godoy Cruz 2290 (C1425FQB) CABA, Buenos Aires, Argentina; Laboratory of Immunoparasitology (LAINPA), Faculty of Veterinary Sciences, National University of La Plata (FCV-UNLP), La Plata 1900, Argentina.
Parasitology international
|January 10, 2024
概括
萨尔科西斯蒂斯 spp. 的一种. 在阿根廷发现了猪感染,在半扩展性养殖中患病率更高. 分子和显微镜分析证实Sarcocystis miescheriana是主要物种,提供了关键的感染数据.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子生物学分子生物学
- 食品安全 食品安全
背景情况:
- 萨尔科西斯蒂斯 spp. 的一种. 它们是显著的细胞内原生虫寄生虫,会影响牲畜.
- 了解猪肉囊的流行率和物种对于动物健康和食品安全至关重要.
研究的目的:
- 来自阿根廷的猪肌肉中识别Sarcocystis物种.
- 根据农业系统来确定感染率.
- 用显微镜和分子技术来表征寄生虫.
主要方法:
- 光显微镜和传输电子显微镜 (TEM) 用于形态分析.
- 聚合酶链反应 (PCR) 针对 18S rRNA 和 coxI 基因进行分子识别.
- 在密集型 (IF) 和半扩展型 (SEF) 养殖系统下,分析了来自561头猪的肌肉样本.
主要成果:
- 通过光显微镜检测,囊炎的总体患病率为24.8%,在SEF (34.6%) 比IF (15.9%) 猪显著高.
- 在47.5%的样本中,PCR检测到18SrRNA;所有测试样本对S. suihominis.是负的.
- 测序和TEM证实了Sarcocystis miescheriana的存在,与已知菌株的100%序列一致性.
结论:
- Sarcocystis miescheriana 是在阿根廷的猪中发现的占主导地位的Sarcocystis物种.
- 半扩展性农业系统与较高的囊炎感染率有关.
- 这项研究提供了关于阿根廷猪中 Sarcocystis 感染率和物种识别的第一个全面数据.
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