相关实验视频

Updated: Jul 6, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

针对RNF12/RLIM E3的染色体向控制了转录反应的控制

Carmen Espejo-Serrano¹, Catriona Aitken¹, Beatrice F Tan²

¹MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, UK.

Life science alliance

|January 10, 2024

概括

E3 泛基因酶 RNF12 向 REX1 转录因子,以调节基因表达. 染色体对RNF12的招募对于这个过程和REX1无处不在至关重要,为转录调节提供了洞察力.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

蛋白质无处不在是生物过程的关键调节者,包括转录.
E3 泛基因酶 RNF12 (RLIM) 泛基化了REX1转录因子,影响了发育基因表达,并与X相关的智力障碍有关.
无处不在影响特定转录结果的机制仍然不完全理解.

研究的目的:

阐明RNF12介导的无处不在调节基因转录的精确机制.
研究RNF12染色体招募与REX1基质的相互作用及其随后的转录控制中的作用.
了解在特定基因组位置控制RNF12活动的调节机制.

主要方法:

染色体免疫沉 (ChIP) 用于识别RNF12基因组结合部位.
对RNF12蛋白域的分析,包括一个基本区域和N端自抑制域.
评估REX1无处不在状态和RNF12依赖的基因表达变化.
位点定向突变发生,以探测RNF12域在染色质招募和催化中的功能.

主要成果:

通过共识序列动机,RNF12被招募到特定的基因组位置,促进与REX1在基因促进器的共同定位.
RNF12染色体的招募是由一个非催化性基本区域介导的,并且涉及一个未表征的N-终端自身抑制机制.
向RNF12染色体对于REX1无处不在和随后对RNF12依赖基因的调节至关重要.

结论:

染色体在调节RNF12-REX1信号轴方面发挥着至关重要的作用.
通过其基本区域和自身抑制域,RNF12对染色质的招募对于其在基因表达编程中的功能至关重要.
这项研究为蛋白质无处不在如何驱动特定的转录反应提供了新的见解.

更多相关视频

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells

Published on: May 10, 2019

相关概念视频

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Co-activators and Co-repressors

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Eukaryotic Transcription Inhibitors

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...