菌 (Faecalibacterium duncaniae A2-165) 调节了基于乙酸盐消耗的丁酸盐合成,铁素吸收和应激反应基因的表达
Sophie Verstraeten1, Séverine Layec1, Sandrine Auger1,2
1Micalis Institute, INRAe, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
Scientific reports
|January 10, 2024
概括
乙酸盐的可用性会影响益生菌Faecalibacterium prausnitzii中的基因表达. 高酸盐增加了酸盐的合成和营养进口,特别是铁通过FeoB,而低酸盐触发了应激反应.
科学领域:
- 微生物学 微生物学
- 肠道微生物组研究研究
- 细菌生理学 细菌生理学
背景情况:
- 菌 (Faecalibacterium prausnitzii) 是一个关键的肠道细菌,参与乙酸代谢.
- 了解酸盐水平如何影响F.prausnitzii基因表达对于其益生菌应用至关重要.
- 关于F. prausnitzii对不同酸盐度的转录反应的知识有限.
研究的目的:
- 为了研究酸盐可用性对F. duncaniae A2-165.5的时转录组的影响.
- 在不同的酸盐条件下,比较早期静止和晚期指数增长阶段的基因表达.
- 为了阐明铁和B族维生素进口途径对乙酸盐的反应的调节.
主要方法:
- 使用RNA测序分析了F. duncaniae A2-165种植物的转录组.
- 培养在低 (3 mM) 和高 (23 mM) 乙酸盐条件下生长.
- 基因表达模式在早期静止和晚期指数增长阶段之间进行了比较.
主要成果:
- 低酸盐诱导了一般的压力反应.
- 高酸盐增加了对丁酸盐合成,维生素B和铁进口基因的表达.
- 在高酸盐下,feoAABC操作子 (FeoB转运体) 显示出强烈的激活,并被过量的铁抑制;feoAB不受影响.
- 与FeoAB不同的是,FeoB在人类便的甲型蛋白质中很丰富.
结论:
- 乙的可用性显著影响了F. prausnitzii的转录组.
- 在高酸盐条件下,FeoB介导的铁运输是F. prausnitzii的关键调节途径.
- 这项研究提供了关于feoB表达的调节及其在人类肠道微生物组中的相关性的见解.
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