揭示非综合症裂唇与或没有裂 palatal 发病因子的失调 lncRNAs 和网络
Caihong Wu1,2,3, Haojie Liu1,2, Zhuorong Zhan1,2
1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.
Scientific reports
|January 10, 2024
概括
这项研究确定了关键的长非编码RNAs (lncRNAs) 和信使RNAs (mRNAs) 参与非综合征裂唇与或没有裂 palates (NSCL/P) 的发展. 这些发现揭示了NSCL/P病变的基础的关键分子网络.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 带有或没有裂的非综合性裂唇 (NSCL/P) 是一种常见的先天性面部形,具有复杂的,不完全理解的遗传起源.
- 长非编码RNAs (lncRNAs) 越来越被认为是基因表达的关键调节者,在发育障碍中可能发挥作用.
研究的目的:
- 识别与NSCL/P相关的失调的lncRNA和信使RNA (mRNA).
- 构建调节网络 (ceRNA, lncRNA-RBP, cis/trans) 以阐明NSCL/P的分子机制.
- 为了验证NSCL/P病变发生的关键分子参与者.
主要方法:
- 整合基因表达总体 (GEO) 基因表达配置文件.
- 竞争性内源RNA (ceRNA),lncRNA-RNA结合蛋白 (RBP) 和lncRNA cis/trans调节网络的构建.
- 使用RT-qPCR验证,并分析人类NSCL/P组织和小鼠 palatal shelves中的蛋白质水平.
主要成果:
- 确定了30个失调的NSCL/P相关的lncRNAs.
- 确定了五个关键分子 (FENDRR,TPT1-AS1,EIF3H,RBBP6,SRSF1) 可能有助于NSCL/P的发病.
- 功能性丰富分析揭示了与NSCL/P中的lncRNA相关网络相关的生物功能和途径.
结论:
- 这项研究全面地绘制了NSCL/P.P.中失调的转录格局.
- 确定了关键的lncRNA和mRNA子网络,这些子网络对于NSCL/P发展至关重要.
- 提供了进一步研究INcRNA和mRNA在NSCL/P中的调节作用的基础.
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