蛋白质组分析确定了突变小尾汉羊粒状细胞中高排卵的独特蛋白质模式
Xiangyu Wang1, Xiaofei Guo2,3, Xiaoyun He1
1State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Animals : an open access journal from MDPI
|January 11, 2024
概括
绵羊的Booroola生育能力 (FecB) 突变通过改变参与新陈代谢的颗粒细胞蛋白来增强排卵. 这导致FecB突变羊的卵细胞成熟和质量得到改善.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 在BMPR1B基因中的Booroola生育能力 (FecB) 突变增加了绵羊的排卵率.
- FecB突变对毛囊成熟的分子机制的确切影响仍然不完全理解.
研究的目的:
- 为了研究与FecB突变相关的颗粒细胞 (GCs) 中的蛋白质变化.
- 确定由FecB影响的关键蛋白质和代谢途径,有助于改变排卵率和卵细胞质量.
主要方法:
- 在野生型 (WW),异合体 (WB) 和同合体 (BB) FecB突变小尾汉羊的GC上使用双重质量标记 (TMT) 技术进行蛋白质组分析.
- 使用LIMMA包装进行统计分析,以识别差异丰富的蛋白质 (p < 0.05).
- 生物信息通路丰富分析 (KEGG,GO) 和多变量分析 (sPLS-DA,Fuzzy C-means) 以确定功能相关的蛋白质.
主要成果:
- 199种蛋白质受到FecB突变的显著影响.
- 丰富的途径包括胆固醇,碳水化合物,氨基酸和谷氨代谢,对于卵细胞成熟至关重要.
- 与野生类型 (WW) 相比,参与胆固醇代谢的蛋白质如ZP2,ZP3 (蛋皮) 和阿波利波蛋白 (APOA1,APOA2,APOA4) 在FecB突变羊 (WB和BB) 中被上调.
- 这些蛋白质对FecB基因型具有很高的区分能力.
结论:
- 在GC中FecB突变增强营养代谢,积极影响卵细胞成熟.
- 在FecB突变体中ZP2,ZP3和APOA1的上调表明了改善卵细胞质量和增加排卵率的机制.
- 这些发现为FecB突变羊的增强生育能力的分子基础提供了新的见解.
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