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对花状细胞的转录组分析揭示了与毛囊发育相关的调控机制

Xubin Du^1,2, Qizhao Zhu¹, Huifang Pian¹

¹Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

Animals : an open access journal from MDPI

|January 11, 2024

概括

这项研究揭示了早期和晚期卵泡颗粒细胞之间的关键基因表达差异,突出了类固醇激素在卵巢卵泡发育和选择中的关键作用.

科学领域:

生殖生物学生殖生物学
分子内分泌学分子内分泌学
基因组学就是基因组学.

背景情况:

卵巢卵泡细胞对于卵巢卵泡的发育和选择至关重要.
了解前层次和前排卵毛囊颗粒细胞的不同功能对于生殖研究至关重要.
基因表达特征提供了洞察细胞分化和功能在卵泡发育过程中.

研究的目的:

为了区分前层次毛囊颗粒细胞 (PHGCs) 和排卵前毛囊颗粒细胞 (POGCs) 的功能.
确定参与卵巢卵泡发育和选择的关键基因.
为了研究类固醇激素在颗粒细胞功能中的作用.

主要方法:

对分别收集的PHGC和POGC进行了RNA测序 (RNA-seq).
差异基因表达分析确定了5273个差异表达基因 (DEG).
定量PCR (qPCR) 验证了16个选定的DEGs的RNA-seq发现.
进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.

主要成果:

总共有5273个DEG被确定,其中2797个在PHGC上调和2476个在POGC下调,与POGC相比.
与类固醇激素合成相关的基因在POGC中显著增加,而与类固醇生成抑制相关的AMH和WT1等基因则减少.
毛囊形态,特别是花质层厚度,随着发育和年龄的增长出现了显著的变化.

结论:

类固醇激素在卵巢卵泡的发育和选择中起着关键作用.
DEGs主要参与代谢过程,细胞功能,核糖体和TGF-β等信号通路.
在毛囊发育过程中,花状细胞组织的形态和功能会动态变化.

关键词:

毛囊的发育毛囊的发育细胞颗粒状颗粒素的细胞.类固醇激素类激素类固醇激素的产生转录组 (transcriptome) 是一个转录组.

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