通过衍生-气相染色学,并列质谱法对蛋中脱酸盐残留物的定量分析
Yali Zhu1,2, Lan Chen2,3, Yawen Guo1,2
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Foods (Basel, Switzerland)
|January 11, 2024
概括
一种新方法使用气体染色学双重质谱法 (GC-MS/MS) 准确检测蛋中的甲基酸盐残留物. 这种经过验证的技术通过高灵敏度和精确度量化药物水平来确保蛋的安全.
科学领域:
- 分析化学 分析化学
- 食品安全科学 食品安全科学
背景情况:
- 德可酸是一种用于家禽的菌抑制剂,引发了人们对蛋等食用产品中的残留物质的担忧.
- 准确的检测方法对于确保食品安全和遵守法规限制至关重要.
研究的目的:
- 开发和验证一种新型的前列导化-气相色谱双重质谱法 (GC-MS/MS) 方法,用于检测各种蛋成分中的脱酸残留物.
- 确定开发的分析方法的灵敏度,准确性和精度.
主要方法:
- 液态-液态提取 (LLE) 和固态提取 (SPE) 用于样品制备.
- 使用皮里丁和酸无水化物的前列衍生.
- 检测和确认通过气体染色学并联质谱法 (GC-MS/MS).
主要成果:
- 经过优化实验条件,对甲可酸检测产生了令人满意的灵敏度.
- 检测极限 (LODs) 范围在1.4-2.4μg/kg之间,量化极限 (LOQs) 范围在2.1-4.9μg/kg之间.
- 平均恢复率为74.3-89.8%,在日内 (1.22-4.78%) 和日间 (1.61-7.54%) 分析中,相对标准偏差 (RSD) 较低.
结论:
- 开发的GC-MS/MS方法对于在蛋中检测脱酸残留物具有敏感性,准确性和精确性.
- 该方法的可行性和实用性通过使用超市真正的蛋样本得到证实,支持其在常规食品安全监测中的应用.
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