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Updated: Jul 6, 2025

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
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在小鼠中枢神经系统中,MEGF8的免疫组合化学和免疫电子微观分布

Kazuhiko Nakadate1, Kiyoharu Kawakami1

  • 1Department of Basic Science, Educational and Research Center for Pharmacy, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose 204-8588, Tokyo, Japan.

Cells
|January 11, 2024
PubMed
概括

多重表皮生长因子类域8 (MEGF8) 在小鼠中枢神经系统 (CNS) 中广泛分布. 这种蛋白质存在于突触和线粒体上,表明它在神经元功能中的作用.

关键词:
E3 无处不在的合酶线粒体中的线粒体.神经元神经元的神经元蛋白质酶体蛋白质组是什么突触突触是指突触中的突触.在任何地方都是无处不在的.

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Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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