相关实验视频

Updated: Jul 6, 2025

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

进口的病毒向在阿尔法介导核进口以阻止天生的免疫力

Olivia A Vogel¹, Jade K Forwood², Daisy W Leung³

¹Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

|January 11, 2024

概括

病毒通过劫持进口蛋白α (IMPA) 蛋白质来阻止抗病毒基因表达. 这种病毒策略竞争性地抑制了基本转录因子的核进口,抑制了宿主.

科学领域:

细胞生物学细胞生物学
病毒学病毒学
免疫学免疫学免疫学

背景情况:

细胞核细胞质流通依赖于进口蛋白质.
进口素α (IMPA) 和进口素β (IMPB) 途径对于对病毒的天生的免疫至关重要.
这条途径输入转录因子 (IRF3,NFκB,STAT1) 来诱导像干扰素 (IFN) 和IFN刺激基因 (ISG) 这样的抗病毒基因.

研究的目的:

审查干扰宿主天生的免疫反应的病毒策略.
要突出病毒如何阻止宿主抗病毒转录因子的核进口.
为了检查具有竞争性抑制进口素α (IMPA) 功能的病毒蛋白.

主要方法:

关于病毒逃避机制的科学文献的综述.
对病毒蛋白与Importin alpha (IMPA) 相互作用的分析.
检查对宿主抗病毒基因表达的影响.

主要成果:

病毒使用各种策略来抵消宿主抗病毒防御.
一种常见的病毒策略包括阻止关键宿主转录因子的核进口.
病毒蛋白结合进口因子α (IMPA),防止转录因子转位和抑制抗病毒基因诱导.

结论:

病毒蛋白利用竞争性抑制,通过向进口因子α (IMPA) 来逃避天生的免疫力.
这种机制有效地抑制了关键的抗病毒基因的上调调节,帮助病毒复制.
了解这些病毒逃避策略对于开发抗病毒疗法至关重要.

关键词:

非洲猪瘟病毒病毒埃博拉病毒埃博拉病毒.冠状病毒冠状病毒的新冠病毒.弗拉维病毒 (flavivirus) 是一种病毒.汉塔病毒汉塔病毒乙型肝炎病毒的感染.人类免疫缺陷病毒-1 免疫逃避免疫逃避在阿尔法进口中.具有天生的免疫力.干扰子干扰子干扰子疫苗病毒病毒病毒病毒病毒.

更多相关视频

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Published on: November 3, 2014

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Published on: November 15, 2024

相关概念视频

Nuclear Localization Signals and Import

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of 2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...

Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Cells of the Innate Immune Response

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...