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来自Poria cocos的两种多糖的免疫调节活性及其机制

Wuxia Zhang¹, Jiaqi He¹, Danping Zheng¹

¹Shanxi Key Laboratory for Modernization of TCVM, Department of Basic Sciences, Shanxi Agricultural University, Jinzhong 030801, China.

Molecules (Basel, Switzerland)

|January 11, 2024

概括

来自Poria cocos的两种多糖,PCWPW和PCWPS,通过增强TNF-α通过曼诺斯受体和NF-κB/MAPK通路的分泌来调节巨细胞的免疫反应,这表明免疫相关疾病治疗的潜力.

科学领域:

菌类学菌类学是指菌类学.
免疫学免疫学免疫学
药理学药理学是一个学科.

背景情况:

包括Poria cocos在内的Polyporaceae真菌是生物活性天然产品的宝贵来源.
之前的研究已经确定了来自Poria cocos的PCWPW和PCWPS多糖类,具有抗氧化和抗抑郁作用.

研究的目的:

研究PCWPW和PCWPS在巨细胞上的免疫调节机制.
阐明参与由这些多糖调节的免疫反应的分子途径.

主要方法:

气色谱-质谱 (GC-MS) 用于多糖的表征.
与酶相关的免疫吸收试验 (ELISA) 用于测量TNF-α分泌.
RNA测序 (RNA-seq) 和RT-qPCR用于分析基因表达.
使用曼诺酶受体 (MR) 和信号通路抑制剂 (NF-κB,MAPK) 的抑制试验.

主要成果:

PCWPW和PCWPS含有1,3-结合的Glcp,并显著增强巨细胞中的TNF-α分泌.
PCWPS调节了与免疫相关的基因表达.
TNF-α的分泌取决于曼诺酶受体 (MR) 的激活.
NF-κB和MAPK信号通路参与了PCWPW/PCWPS诱导的TNF-α产生.

结论:

PCWPW和PCWPS对巨细胞具有免疫调节作用.
这些多糖类通过MR结合和随后的NF-κB/MAPK信号通路的激活来调节TNF-α的表达.
PCWPW和PCWPS显示出对免疫相关疾病的治疗剂的潜力.

关键词:

在 MAPK 信号通道中.通过NF-κB信号通路传递信号.波利亚可可斯的多糖化物.这是一种免疫调节剂.曼诺糖受体的受体是什么?

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