液体染色学-高分辨率质谱学 (LC-HRMS) 指纹和化学测量用于咖啡分类和认证
Nerea Núñez1,2, Javier Saurina1,2, Oscar Núñez1,2,3
1Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain.
Molecules (Basel, Switzerland)
|January 11, 2024
概括
这项研究开发了一种液体染色学高分辨率质谱法,以验证咖啡的来源,品种和烤度. 该技术有效地识别了改和分类咖啡样本,确保了饮料质量并防止欺诈行为.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 化学测量 化学测量 化学测量
背景情况:
- 咖啡等天然产品的造越来越多,因此需要强有力的认证方法.
- 确保咖啡的来源,品种和烤度对于消费者信任和公平贸易至关重要.
研究的目的:
- 开发和验证一种非向液体染色学高分辨率质谱法 (LC-HRMS) 方法,用于咖啡的表征和分类.
- 为了验证咖啡品种 (阿拉伯咖啡与罗布斯塔咖啡),种植地区和度的真实性.
- 检测和量化咖啡样本中的改.
主要方法:
- 分析了306个咖啡样本,使用C18逆相LC加HRMS和负模式的电喷气电离.
- 使用热水提取分析物 (咖啡造).
- 化学测量分析包括主要成分分析 (PCA) 和部分最小平方区分分析 (PLS-DA) 进行分类,以及部分最小平方回归 (PLS) 进行伪造量化.
主要成果:
- 通过LC-HRMS方法,成功地根据品种,地区和烤度对咖啡样品进行了特征和分类.
- 化学测量模型在区分不同咖啡群体方面表现出很高的准确性.
- 该方法准确地量化了伪造剂的含量,降至15%,校准和预测误差较低.
结论:
- 拟议的LC-HRMS方法与化学测量相结合,是咖啡认证和伪造检测的强大工具.
- 这种方法可以保障全球市场上咖啡产品的质量和完整性.
- 该研究提供了一种可靠的方法来确保咖啡的真实性,打击欺诈行为.
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