通过使用两个独立的测量策略,认证新的丰富酵母和甲补充剂参考材料
Xiao Li1,2, Ling Shi2, Panshu Song2
1School of Ocean Sciences, China University of Geosciences (Beijing), Beijing 100083, China.
Molecules (Basel, Switzerland)
|January 11, 2024
概括
富含的酵母将无机转化为甲,是一种安全和可吸收的形式. 这项研究建立了经过认证的参考材料,用于准确测量酵母和补充剂中的氨酸.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 食品科学 食品科学 食品科学
背景情况:
- 是必不可少的微量元素,但它的化学形式决定了它的毒性和生物可用性.
- 富含的酵母将无机转化为甲,是一种安全且可生物利用的形式.
- 甲素经常被纳入富含的补充剂中.
研究的目的:
- 开发和认证用于准确量化二甲的参考材料.
- 在复杂矩阵中验证有机物种化的测量策略.
- 为了支持在丰富食品和补充剂的生产质量控制.
主要方法:
- 使用高性能液体染色学与诱导合等离子体质谱学 (HPLC-ICP-MS) 相结合.
- 雇佣的高性能液体染色学与电子喷射电离-质谱/质谱相结合 (HPLC-ESI-MS/MS).
- 应用了两种不同的质谱技术进行物种分析.
主要成果:
- 在丰富的酵母粉中确定了甲的质量分数为718毫克SeMet kg-1 (HPLC-ICP-MS) 和715毫克SeMet kg-1 (HPLC-ESI-MS/MS).
- 使用这两种方法在补充剂中测量出一致的59毫克SeMet kg-1的甲质量分数.
- 认证的参考值:酵母为716毫克/公斤,补充剂为59毫克/公斤,与此相关的不确定性.
结论:
- 确立了酵母和补充剂中氨酸的可靠候选参考材料.
- 证明了HPLC-ICP-MS和HPLC-ESI-MS/MS在有机物种化中的有效性.
- 为准确确定食品矩阵中的有机提供了必不可少的工具.
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