在Clostridioides difficile的环境分离物体内进行体携带和遗传多样性
1Department of Microbiology-Biotechnology, Faculty of Technology, University of Applied Sciences Emden/Leer, 26723 Emden, Germany.
International journal of molecular sciences
|January 11, 2024
概括
环境Clostridioides difficile菌株携带多种不同的prophages,其中一些具有CRISPR阵列. 这些集成的prophages可能会影响细菌生物学和健康状况.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 细菌生物学的生物学
背景情况:
- 在全球范围内,Clostridioides difficile会导致与抗生素相关的腹.
- 细菌被探索为替代疗法.
- 在环境C. difficile中对食原体运输的研究不足.
研究的目的:
- 调查环境C. difficile分离物中综合性菌体的流行率和遗传多样性.
- 在预兆区域内识别CRISPR-Cas系统.
- 分析已识别的prophages的遗传学关系.
主要方法:
- 对166个环境C. difficile分离物的基因组分析.
- 使用PHASTER网络服务器进行预测.
- 使用CRISPRCasFinder进行CRISPR-Cas系统识别.
- 基于菌体相关基因 (TerL,LysM) 的遗传学分析.
主要成果:
- 鉴定了372个完整的prophages,其中phiCDHM1和phiCDHM19占主导地位.
- 发现了两个新型的细菌病毒:phiSM101和phivB_CpeS-CP51类的Clostridium菌体.
- 发现prophage类型和序列类型 (STs) /ribotypes之间的相关性.
- 观察到大多数prophages携带CRISPR阵列和辅助基因 (Agr,Abi).
结论:
- 环境C. difficile菌株含有各种各样的菌体.
- 前体载体,遗传多样性和CRISPR阵列可能会影响宿主菌株的生物学,生活方式和健康状况.
- 这些发现有助于理解环境环境中的菌体与宿主相互作用.
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