相关实验视频

Updated: Aug 1, 2026

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Published on: July 11, 2017

基于分子动力学模拟的单颗粒图像和子电图数据集提取连续形态景观的MDSPACE和MDTOMO软件:ContinuousFlex软件包的最新发展

Rémi Vuillemot¹, Mohamad Harastani¹, Ilyes Hamitouche¹

¹IMPMC-UMR 7590 CNRS, Sorbonne Université, MNHN, 75005 Paris, France.

International journal of molecular sciences

|January 11, 2024

概括

这项研究引入了新的软件MDSPACE和MDTOMO,用于分析冷电子显微镜 (cryo-EM) 数据. 它揭示了生物分子的连续构造景观,为其动态结构提供了更完整的图像.

科学领域:

结构生物学结构生物学
生物物理学的生物物理.
计算生物学计算生物学

背景情况:

电子显微镜 (cryo-EM) 提供了对生物分子复合体的3D结构洞察力.
标准的冷EM方法通常只捕获离散的形状状态,限制了对动态转换的理解.
持续的形状变化对生物分子功能至关重要,但通过传统的冷电磁分析难以解决.

研究的目的:

介绍一个易于使用的软件,用于分析冷EM数据,以揭示连续的形状景观.
为了使生物分子动力学在体外和在现场使用冷EM的全面分析.
为探索分子复合体的完整形态变异性提供一个计算框架.

主要方法:

在ContinuousFlex包中开发和实施MDSPACE和MDTOMO软件.
将分子动力学模拟与冷EM数据分析 (单颗粒图像和子图) 的整合.
利用Scipion软件作为运行新分析方法的平台.

主要成果:

该软件可以从冷EM数据生成连续的形状景观.
能够对离散状态之外的生物分子动态进行详细分析.
提供了更完整的分子复合体的结构异质性的表现.

结论:

提出的软件在分析冷EM数据以了解生物分子动态方面取得了重大进展.
MDSPACE和MDTOMO为研究对形状灵活性的研究人员提供了强大的工具.
这种方法增强了冷EM用于阐明生物分子的功能机制的实用性.

关键词:

连续灵活的连续的 MDSPACE MDSPACE MDSPACE 这是一个很大的空间.在 MDTOMO 里面.斯基皮翁 (Scipion) 是一个天.生物分子复合体的生物分子复合体.构成性的异质性构成性的异质性连续的构造性景观.化电子显微镜技术的电子显微镜软件软件软件软件软件用户友好的图形界面.

更多相关视频

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

相关概念视频

Molecular Models

Molecular Models

Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...