相关实验视频
Updated: Jul 5, 2025

09:09
Gene Expression Analyses in Human Follicles
Published on: February 17, 2023
1.2K
在人类产卵/卵泡发育过程中对基因表达动态的概述
Bastien Ducreux1, Lucile Ferreux2,3, Catherine Patrat2,3
1Université Bourgogne Franche-Comté-Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231, 2 Rue Angélique Ducoudray, F-21000 Dijon, France.
International journal of molecular sciences
|January 11, 2024
概括
人类卵细胞的成熟涉及到对早期发育至关重要的动态基因表达变化. 这项研究解读了这些转录基因转移,确定了关键基因及其在 oogenesis 期间的调节,直到第 II 阶段的转录基因转移.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 发育生物学 发展生物学
背景情况:
- 卵细胞成熟是一个复杂的过程,由精确的转录基因调节控制.
- 了解这些母体mRNA动态对于人类生殖成功至关重要.
- 以前的研究还没有完全解码人类 oogenesis 中的转录组事件.
研究的目的:
- 重新分析人类单卵细胞RNA-seq数据,以了解 oogenesis 期间的转录组变化.
- 确定关键的基因和调控事件,确保卵细胞的能力,直到第二阶段的转化阶段.
- 揭示与影响卵细胞质量的环境因素相关的转录组变化.
主要方法:
- 重新分析现有的人类单卵细胞RNA-seq数据集.
- 运用轨迹分析 (伪物质时间) 和元分析.
- 在 oogenesis 过程中具有显著表达变异的基因的识别和特征.
主要成果:
- 揭示了从原始阶段到第二阶段的卵子细胞发育的基本转录基因要求.
- 鉴定了具有显著表达变化的基因,并描述了它们的时间调节.
- 揭示了能量/线粒体转录的下调和转化/RNA过程转录在胚芽囊到MII过渡期间的保存.
- 发现了对母亲年龄,PCOS,冷保存和体外成熟敏感的某些基因.
结论:
- 这项研究提供了人类 oogenesis 期间的转录基因变化的全面地图.
- 已识别的转录组景观对于卵细胞的成熟和能力至关重要.
- 这些知识可以帮助识别卵细胞成熟失败的原因,并改善辅助生殖技术.
相关概念视频
Oogenesis
1.6K
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
1.6K
Folliculogenesis
785
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
785
Hormonal Control of the Ovarian Cycle
518
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
518
Ovarian Cycle
1.2K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
1.2K
Structure of a Gene
12.6K
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
12.6K
Hormonal Regulation of the Menstrual Cycle
396
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
396

