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化适配器RNA出口蛋白在Lys 381被一种类似甲基转移酶的21C (METTL21C) 甲基化

Meiling Ke¹, Xiaoke Yu¹, Yuanyuan Sun¹

¹School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China.

International journal of molecular sciences

|January 11, 2024

概括

肉METTL21C (甲基转移酶类21C) 通过阻断细胞循环来抑制DF-1细胞增殖. 它调解了包括PHAX在内的关键蛋白质的氨酸三甲基化,影响细胞生长和发育.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
禽畜科学禽畜科学禽畜科学

背景情况:

甲基转移酶类21C (METTL21C) 是一种非质甲基转移酶,主要在哺乳动物中进行研究.
在家禽中METTL21C功能,特别是胚胎纤维细胞 (DF-1细胞) 的研究是有限的.
METTL21C调解氨酸 (Lys) 二甲基化和三甲基化,这是关键的翻译后修改.

研究的目的:

研究肉METTL21C (chMETTL21C) 在DF-1细胞增殖中的作用.
阐明chMETTL21C调节细胞周期进展和生长的分子机制.

主要方法:

使用流细胞计和定量实时PCR (qPCR) 来评估细胞增殖.
蛋白质组分析被用来识别受METTL21C过度表达影响的蛋白质.
免疫沉 (co-IP) 和液态染色体质谱学 (LC-MS/MS) 用于确认特定的甲基化事件.

主要成果:

过度表达chMETTL21C导致细胞周期在G1/S阶段停止,抑制DF-1细胞增殖.
蛋白质组分析表明,chMETTL21C通过调解PHAX,NDPKs,eEF1A和Invs等蛋白质的lysine三甲基化来抑制增殖.
在Lys-381中通过METTL21C介导的PHAX甲基化被证实参与调节DF-1细胞增殖.

结论:

肉METTL21C通过阻止细胞循环,在抑制DF-1细胞增殖方面发挥着重要作用.
该研究确定了chMETTL21C针对氨酸三甲基化所针对的特定的与增殖相关的蛋白质,提供了机理性的见解.
这项研究为了解METTL21C的甲基化功能及其对细胞生长和发育的影响提供了基础.

关键词:

一个DF-1细胞.这是LC-MS/MS.在METTL21C中,您可以使用METTL21C.细胞的增殖细胞的增殖.氨酸三甲基化是氨酸的三甲基化.

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