代谢学和转录学分析黄素缓解奥克拉托克辛A诱导的肝毒性
Peng Hui1, Xianrui Zheng2, Jiao Dong1
1Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
International journal of molecular sciences
|January 11, 2024
概括
黄素 (CUR) 通过改变肝脏基因和代谢物配置文件,减轻了Ochratoxin A (OTA) 的毒性. 潘托酸 (PA) 补充剂进一步降低了OTA诱导的氧化应激和亡,突出了CUR.
科学领域:
- 毒理学 毒理学 毒理学
- 代谢学 代谢学 代谢学
- 文字转录学 (Transcriptomics) 是一个学科.
- 营养生物化学 营养生物化学
背景情况:
- 毒素A (OTA) 是一种普遍存在的真菌毒素,具有显著的人类和动物健康风险.
- 黄 (CUR) 是一种来自黄的化合物,具有健康益处,并显示出减轻真菌毒素毒性的潜力.
- 在CUR对OTA毒性的保护作用背后的分子机制尚未完全理解.
研究的目的:
- 研究CUR对小鼠肝脏中OTA诱导毒性的影响.
- 确定关键的分子调节剂和参与CUR和OTA影响的生物过程中的代谢物.
- 阐明CUR对OTA毒性的保护机制.
主要方法:
- 代谢和转录基因分析对OTA暴露的小鼠肝脏进行,并补充了CUR.
- 综合生物信息学分析被用来识别受影响的途径和关键代谢物.
- 进行了涉及活性氧物种,亡标记物 (BAX,BCL2) 和信号通路 (pERK1/2) 的机制研究.
主要成果:
- CUR减轻了OTA引起的对身体生长和肝功能的不良影响.
- 补充CUR显著改变了1584个基因和97个代谢物的表达.
- 关键的丰富途径包括阿拉基酸,纯氨酸和胆固醇代谢.
- 潘托酸 (PA) 被确定为一种关键的代谢物,通过调节BAX,BCL2和pERK1/2水平来减轻OTA诱导的氧化应激和亡.
结论:
- 通过全面调节肝脏转录和代谢形状,CUR减轻了OTA的毒性.
- 潘托酸在CUR对OTA诱导的细胞损伤的保护作用中起着重要作用.
- 这些发现支持CUR在食品和料中的潜在应用,以防止OTA毒性.
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