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优化彗星测试基于体外DNA修复测试对胎盘组织的测试:一项试点研究与前结核患者
Anastasiya Mircheva1, Philippe Vangrieken2, Salwan Al-Nasiry3
1Department of Pharmacology and Toxicology, School for Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands.
International journal of molecular sciences
|January 11, 2024
概括
我们优化了对胎盘组织的DNA修复试验,在子宫前中发现了下基切除修复 (BER) 活性. 这种DNA修复洞察力可以促进对产前疾病的理解.
科学领域:
- 生物化学 生物化学
- 遗传学 是一个遗传学.
- 生殖生物学 生殖生物学
背景情况:
- 彗星测定是测量细胞中DNA修复活动的标准工具.
- 胎盘是一种有价值的,非侵入性组织,用于研究生命早期的暴露和产前条件.
- 研究胎盘组织中的DNA修复机制可以阐明产前疾病的分子基础.
研究的目的:
- 为了优化彗星试验,量化人类胎盘组织中的基切除修复 (BER) 活性.
- 建立一种可靠的方法,用于生物监测生命早期的暴露,并使用胎盘组织调查产前疾病.
- 为了评估与健康对照相比,胎盘前怀孕的胎盘组织中的BER活性.
主要方法:
- 胎盘提取物的最佳蛋白质度为2 mg/mL,用于精确的DNA损伤识别和切口.
- 确定在优化协议中,阿菲迪科林不需要减少非特异性切口.
- 确认采样到分析间隔长达70分钟不会影响BER活动测量.
主要成果:
- 与对照组 (n=9) 相比,在妊娠前 (PE) 的胎盘组织中观察到明显较低的基切除修复 (BER) 活性 (n=11).
- 在PE胎盘中,BER相关基因的表达减少.
- 在从PE怀孕的胎盘组织中检测到DNA氧化水平的增加.
结论:
- 优化的彗星测定协议允许在人类胎盘组织中确定BER活性.
- 减少BER活性和增加DNA氧化与先兆子有关.
- 需要进一步的前性研究来探索BER在孕前的发展中的作用.
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