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开发用于癌症免疫治疗的复合性瘤性rVSV-mIL12-mGMCSF

Anastasia Ryapolova¹, Ekaterina Minskaia¹, Nizami Gasanov¹

¹Department of Gene Therapy, Sirius University of Science and Technology, Olympic Avenue, 1, 354340 Sochi, Russia.

International journal of molecular sciences

|January 11, 2024

概括

瘤解毒病毒 (OVs) 在癌症治疗方面表现有前途. 一种表达IL-12和GM-CSF的新型重组VSV部分抑制了黑色素瘤瘤的生长,并改善了小鼠的生存率.

科学领域:

瘤治疗性病毒疗法
免疫治疗是一种免疫疗法.
癌症研究癌症研究

背景情况:

瘤病毒 (OV) 选择性地向并摧毁癌细胞,同时刺激抗瘤免疫力.
膀性口腔炎病毒 (VSV) 是一个有前途的平台,因为它缺乏先前存在的免疫力和易于基因操纵.
通过输送细胞因子来增强OV治疗可以提高疗效并减少全身毒性.

研究的目的:

评估一种新型重组VSV (rVSV) -mIL12-mGMCSF与IL-12和GM-CSF共同表达的小鼠,以增强抗癌作用.
评估内rVSV给药对黑色素瘤瘤生长和动物生存的影响.

主要方法:

在C57BL/6小鼠中注射rVSV-mIL12-mGMCSF和rVSV-dM51-GFP的内注射,这些小鼠患有皮下B16-F10黑色素瘤.
在一项试点研究中,两次病毒注射间隔4天.
监测了瘤生长抑制和生存率.

主要成果:

两种rVSV的治疗导致部分抑制瘤生长,瘤生长抑制指数 (TGII) 约为40%.
与对照组相比,治疗组中的动物的生存率增加了.
该研究表明,通过rVSV共同表达IL-12和GM-CSF具有抗癌作用的潜力.

结论:

重组VSV被设计为共同表达IL-12和GM-CSF显示出作为瘤性黑色素瘤免疫疗法的潜力.
通过rVSV输送这些细胞因子的内输送提供了一种有针对性的方法,以增强抗瘤免疫力并改善生存率.
用优化的设计和更高的病毒剂量进行进一步的研究是有必要的,以调查免疫细胞群体动态.

关键词:

抗癌疗法是一种抗癌治疗.黑色素瘤是一种黑色素瘤.瘤学在瘤学方面.球性病毒 (OV)膀性口腔炎病毒 (VSV) 病毒

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