过度的振荡会导致多精子症,但可能允许小鼠卵子正常发育
Mio Fukuoka1,2, Woojin Kang2, Daiki Katano2
1Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
International journal of molecular sciences
|January 11, 2024
概括
多精子症,或多个精子受精卵,即使在离子 (Ca2+) 振荡受损的情况下,也可以在小鼠中发生. 这些老鼠蛋仍然可以正常发育,这表明与非哺乳动物蛋中看到的多种子症相似的弹性.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 细胞生理学 细胞生理学
背景情况:
- 非哺乳动物卵中的多精子导致离子 (Ca2+) 激增,并选择性精子核进入正常胚胎生成.
- 在人体体体外受精中多精子症的后果仍然不清楚.
- 哺乳动物卵中的多种子和离子 (Ca2+) 振荡之间的关系在很大程度上是未知的.
研究的目的:
- 为了研究受损离子 (Ca2+) 振荡诱导对小鼠卵中的多精子症的影响.
- 为了确定小鼠卵在多精子症的条件下是否能经历正常的胚胎形成,而多精子症是由 Ca2+ 振荡的缺陷引起的.
主要方法:
- 使用的小鼠精子缺乏异粒体酸盐合成酶 (eCS),一个Ca2+振荡诱导剂,产生eCS-knockout (eCS-KO) 精子.
- 在Zona pellucida (ZP) -free和ZP-intact小鼠卵中检查了多种子症的发病率.
- 评估了经过eCS-KO精子受精后产生的卵的发育能力.
主要成果:
- 缺乏eCS的精子表现出延迟的Ca2+振荡启动.
- 发现高精子度可以使Ca2+振荡启动正常化.
- 在ZP完好无损和ZP无损的卵子中,eCS缺陷显著增加了多精子症.
- 使用eCS-KO精子进行受精,产生了可行的eCS-KO男性后代.
结论:
- 在多精子条件下,老鼠卵可能会正常发育,即使Ca2+振荡受到损害.
- 这一发现表明哺乳动物卵发育对多精子的某种程度的弹性,反映了非哺乳动物物种的观察结果.
- 这项研究强调了精子因素,Ca2+信号传递和哺乳动物繁殖中的卵激活之间的复杂相互作用.
相关概念视频
01:38Fertilization
71.0K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
71.0K
02:07Oogenesis
63.7K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.7K
01:57Meiosis II
183.4K
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
183.4K
01:27Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K