相关实验视频
Updated: Jul 5, 2025
09:03
Manufacture and Drug Delivery Applications of Silk Nanoparticles
Published on: October 8, 2016
15.9K
尺寸调整的纳米颗粒的细胞内贩运用于药物输送
Sara Gimondi1,2, Helena Ferreira1,2, Rui L Reis1,2
13B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal.
International journal of molecular sciences
|January 11, 2024
概括
较小的聚合物纳米颗粒 (NP) 显示出优异的药物输送和癌细胞减少. 这项研究强调,即使是NP的微小尺寸变化也会显著影响治疗疗效和细胞吸收.
科学领域:
- 纳米医学是一种纳米医学.
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 聚合物纳米粒子 (NP) 在纳米医学中对于药物输送至关重要.
- 细胞内NP的贩运还没有完全理解,这限制了优化.
- NP大小是一个影响性能的关键参数.
研究的目的:
- 调查20纳米尺寸差异对NP药物输送和细胞内贩运的影响.
- 为了比较50nm和70nm聚乙烯糖醇) 甲基乙烯基乙烯-块-聚乙烯-乳酸盐-co-glycolide (PLGA-PEG) NPs.
- 通过使用多克索鲁比 (Dox) 来评估乳腺癌细胞中的NP性能.
主要方法:
- 将PLGA-PEGNP定制为精确的大小 (50nm和70nm).
- 封装 doxorubicin (Dox) 作为一种模型抗癌药物.
- 评估NP吸收和细胞内定位通过乳腺癌细胞内基因体和溶酶体的免疫染色.
主要成果:
- 50 nm NPs显示癌细胞代谢活动的降低效率高于70 nm NPs.
- 较小的NP (50nm) 表现出较早和更高的细胞吸收.
- 观察到对NP疗效的时间和度依赖的影响.
结论:
- NP的大小显著影响治疗效果,即使是小的变化.
- 优化NP大小对于提高药物输送和抗癌疗效至关重要.
- 与70nmNP相比,50nmPLGA-PEGNP代表了更有效的乳腺癌治疗纳米配方.
相关概念视频
01:23Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport
531
Drugs need to permeate cell membranes to reach their target sites after administration. Orally administered drugs must transcend intestinal epithelial membrane barriers to infiltrate the systemic circulation. Drugs with a molecular weight of less than 500 Daltons diffuse through gaps between neighboring cells, called paracellular pathways.
However, most drugs use the transcellular route, traversing directly through the cell membranes via two mechanisms: passive and active transport. Passive...
However, most drugs use the transcellular route, traversing directly through the cell membranes via two mechanisms: passive and active transport. Passive...
531
01:44Introduction to Membrane Traffic
7.1K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.1K
01:24Cellular Membranes and Drug Transport
560
Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
560