通过批量发酵,通过Aureobasidium melanogenum ZH27的新型真菌进行Pullulan的超级生产
Qin-Qing Wang1,2, Jia Lin1, Qian-Zhi Zhou1
1Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Zhuhai 519082, China.
International journal of molecular sciences
|January 11, 2024
概括
一种新型的Aureobasidium melanogenum菌株ZH27显著增强了pullulan的生产. 优化的条件导致了高产量,细胞形态和基因表达的变化解释了增加的外聚糖产量.
科学领域:
- 微生物学和生物技术
- 生物化学和分子生物学
背景情况:
- 普鲁兰是一种微生物外聚糖,在食品,生物医学和化品行业有多种应用.
- 现有的野生型菌株和突变菌株在pullulan生产产量和遗传稳定性方面表现出局限性,需要发现新的菌株.
研究的目的:
- 发现和描述一种具有增强的普鲁兰生产能力的新型微生物菌株.
- 优化种植条件,以最大限度地提高鉴定品种的普鲁兰产量.
- 调查导致过度生产的潜在机制,包括细胞形态和基因表达,负责过度生产.
主要方法:
- 隔离和鉴定了一种新型的产生普卢兰的菌株,Aureobasidium melanogenum ZH27.7.
- 使用一次性一个因素方法优化发酵条件.
- 在优化和初始条件下分析细胞形态和基因表达特征.
主要成果:
- 菌株ZH27在最佳条件下实现了115.4±1.82g/L的高普卢兰标位,生产率为0.87g/L/h.
- 与初始条件相比,优化条件导致pullulan标位增加105%.
- 在高普卢兰积累期间观察到具有主要大真空的胀细胞,以及与普卢兰合成和副产品形成相关的上调基因.
结论:
- Aureobasidium melanogenum ZH27 是一个有前途的候选人,用于工业生产的pullulan.
- 细胞形态,特别是带有大真空的胀细胞和高调的基因表达是超产量的关键因素.
- 副产品的积累也可能有助于增强的pullulan合成.
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