CBP60b在植物免疫力中发挥了积极和消极的作用
Mei-Yan Ye1, Hu-Jiao Lan1, Jian-Zhong Liu1,2
1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
International journal of molecular sciences
|January 11, 2024
概括
豆类卡尔莫杜林结合蛋白60b (GmCBP60b) 调节植物免疫力. 在大豆和烟草中抑制或过度表达GmCBP60b引发了自身免疫力和对病原体的增强抵抗力,表明功能得到保护.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物免疫力 植物免疫力
- 转录因子 转录因子
背景情况:
- 卡尔莫杜林结合蛋白60b (CBP60b) 是一个转录因子家族成员.
- 在阿拉比多普西斯中,AtCBP60b调节了生长,发育和免疫反应.
- CBP60b主要研究在阿拉比多普西斯,在作物物种上进行的研究有限.
研究的目的:
- 调查GmCBP60b在大豆免疫力中的作用.
- 探索CBP60b在不同植物物种中的保存功能.
- 了解GmCBP60b在植物防御中的调控机制.
主要方法:
- 豆斑病毒 (BPMV) 介导的基因沉默 (BPMV-VIGS) 用于沉默大豆中的GmCBP60b.1/2.
- 生产过基因烟草系列,过度表达GmCBP60b.1.1.
- 与GmSARD1促进剂-露西法酶记者系统进行过渡性联合表达测试.
主要成果:
- 在大豆中抑制GmCBP60b.1/2导致化,并增强对大豆马赛克病毒 (SMV) 和Pseudomonas syringae pv.的耐药性. 甘氨酸 (Psg) 的使用.
- 在烟草诱导的自身免疫中,GmCBP60b.1的过度表达,包括自发细胞死亡,增加PATHOGENESIS-RELATED (PR) 基因表达,升高的酸 (SA) 水平,以及对Pseudomonas syringae pv.增强的耐药性. 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄 DC3000 (Pst DC3000). 番茄
- GmCBP60b.1激活了GmSARD1促进体,这表明通过 (A/T) AATT动机直接进行转录调节.
结论:
- 在调节大豆免疫反应方面,GmCBP60b.1/2具有双重作用 (正和负).
- 在大豆和烟草之间,CBP60b在免疫中的功能保持不变.
- GmCBP60b.1 是GmSARD1的转录激活剂,有助于植物防御.
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