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血循环RNA 0005567作为风湿性关节炎疾病活动的潜在标记物
Marek Cieśla1, Dorota A Darmochwal-Kolarz1, Konrad Kwaśniak2
1Institute of Medical Sciences, College of Medical Sciences, University of Rzeszow, 35-959 Rzeszow, Poland.
International journal of molecular sciences
|January 11, 2024
概括
循环RNAs (circRNAs) 显示出可能成为类风湿性关节炎 (RA) 的生物标志物. 在RA患者中,高水平的circ_0005567与疾病活性相关,这表明其用于监测RA进展.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 循环RNAs (circRNAs) 是稳定的非编码RNA分子,由反向拼接形成.
- 它们的稳定性使得它们与线性RNA相比成为有前途的生物标志物.
- 类风湿性关节炎 (RA) 是一种慢性自身免疫性疾病,需要有效的生物标志物来监测疾病活动.
研究的目的:
- 为了研究特定的circRNAs与风湿性关节炎 (RA) 患者的疾病活性之间的关联.
- 评估circ_0005567作为RA疾病活动的潜在生物标志物.
主要方法:
- 在45名RA患者和26名健康对照 (HCs) 中量化circ_0005567的血度.
- 根据使用DAS-28-ESR得分 (高活性与缓解) 的疾病活性对RA患者的分类.
- 使用SW982细胞系进行体外研究,以探索circ_0005567的生物功能,包括其与miR-194-5p和mRNA表达的相互作用.
主要成果:
- 与HC相比,RA患者中的circ_0005567度显著增加 (p=0.017).
- 与对照组相比,具有高RA疾病活性的患者表现出更高的circ_0005567水平 (p=0.015).
- 在细胞模型中,circ_0005567与miR-194-5p相关,并影响了与细胞增殖相关的mRNA表达.
结论:
- 在RA患者中,血水平升高,与疾病活性相关.
- circ_0005567可以作为一种新的潜在生物标志物,用于评估类风湿性关节炎的疾病活性.
- 需要进一步研究circ_0005567在RA病变发生过程中的功能作用.
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