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代谢学在辅助生殖技术中的整合,用于超越形态动力学分析的增强胚胎选择
Soraia Pinto1, Bárbara Guerra-Carvalho2, Luís Crisóstomo3
1Centre for Reproductive Genetics Alberto Barros, 4100-012 Porto, Portugal.
International journal of molecular sciences
|January 11, 2024
概括
将代谢学与形态动力学相结合,可以改善辅助生殖技术 (ART) 的胚胎质量评估. 特定的代谢物如酸盐和谷氨酸可以预测胚胎活力,提高成功怀孕率.
科学领域:
- 生殖生物学 生殖生物学
- 生物化学 生物化学
- 辅助生殖技术 (ARTs) 是一种辅助生殖技术.
背景情况:
- 在ART中胚胎质量评估依赖于主观形态评估,导致不一致.
- 需要进行客观评估,以改善胚胎转移的选择,并提高成功率.
研究的目的:
- 将胚胎代谢学与形态动力学结合起来,以便对胚胎质量进行更客观的评估.
- 确定能预测胚胎质量和发育潜力的代谢生物标志物.
主要方法:
- 在ICSI后的第3天和第5天从55个胚胎中收集了培养基.
- 根据形态动力学分类胚胎为好,滞后或坏.
- 使用1H-NMR.分析了代谢物变异 (pyruvate,乳酸盐,氨酸,谷氨酸,酸盐,甲酸盐).
主要成果:
- 在培养的前3天的代谢物差异预测了第5天的胚胎质量.
- 酸盐,氨酸,谷氨酸和酸盐是关键的区分因素.
- 好和滞后的胚胎出口了pyruvate和glutamine,而坏的胚胎则消耗了它们,表明代谢活性较低.
结论:
- 代谢学与形态动力学相结合,提供了更全面和客观的胚胎质量评估.
- 酸盐和谷氨酸是预测胚胎活力的潜在生物标志物.
- 通过这种综合方法改善胚胎选择可以提高ART成功率和妊娠结果.
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