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细胞表面GRP78-向的化学抗原受体T细胞消除肺癌瘤外移植

Shijie Wang¹, Wenwen Wei¹, Yuncang Yuan¹

¹Department of Targeting Therapy & Immunology and Laboratory of Animal Tumor Models, Cancer Center and State Key Laboratory of Respiratory Health and Multimorbidity and Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.

International journal of molecular sciences

|January 11, 2024

概括

针对细胞表面葡萄糖调节蛋白78 (csGRP78) 的化学抗原受体-T (CAR-T) 细胞疗法在肺癌中表现有前途. 这种新疗法在体内有效地消除了肺部瘤,没有复发或器官损伤.

科学领域:

在瘤学瘤学.
免疫治疗是一种免疫疗法.
细胞生物学细胞生物学

背景情况:

肺癌的生存率很低,尽管目前的治疗方法.
迫切需要像CAR-T细胞免疫疗法这样的新疗法.
细胞表面葡萄糖调节蛋白78 (csGRP78) 是一种潜在的癌症标,在肺癌细胞上表达,但不是正常细胞.

研究的目的:

研究csGRP78作为肺癌的治疗点.
开发和评估用于肺癌治疗的csGRP78向的CAR-T细胞.

主要方法:

在肺癌细胞系和癌症干细胞类细胞中检测到csGRP78表达.
构造的csGRP78向的CAR和转导的T细胞.
评估了CAR-T细胞在体外和体内对肺癌细胞和异种移植的疗效.

主要成果:

csGRP78在肺癌细胞和癌症干细胞上显著表达.
csGRP78 CAR-T细胞在体外显示出强大的杀死肺癌细胞和干细胞类细胞,与干扰素γ释放.
csGRP78 CAR-T细胞在体内根除了肺瘤异体移植,没有复发或观察到器官毒性.

结论:

csGRP78是肺癌的可行的治疗标.
csGRP78 CAR-T细胞代表了对肺癌的有前途的新免疫疗法,具有有利的安全性.

关键词:

在CAR-T细胞中.在 csGRP78 中使用.免疫疗法免疫疗法肺癌是一种肺癌.

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