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在Schizosaccharomyces pombe中,cytoophidia会影响细胞周期和大小
Ruolan Deng1,2,3, Yi-Lan Li1,2,3, Ji-Long Liu1,4
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
International journal of molecular sciences
|January 11, 2024
概括
丁三酸盐合成酶 (CTPS) 的细胞是细胞增殖的关键. 分裂酵母中CTPS细胞的丧失延长了G2阶段,并增加了细胞大小,影响了细胞循环的控制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 丁三酸盐合成酶 (CTPS) 是一种保存的酶,形成称为cytoophidia的动态丝状结构.
- 细胞菌体参与各种细胞过程,但它们在细胞增殖中的确切作用仍然不完全理解.
- Schizosaccharomyces pombe 作为一个强大的模型生物体,用于研究细胞生物学的基本方面,包括细胞循环调节.
研究的目的:
- 使用裂变酵母模型系统研究CTPS细胞在细胞增殖中的功能.
- 阐明CTPS细胞菌群影响细胞周期进展和细胞大小的分子机制.
- 确定涉及CTPS细胞的潜在遗传相互作用和调节途径.
主要方法:
- CTPS的局部导向突变发生,以破坏细胞的形成 (例如,His359Ala突变).
- 对CTPS蛋白水平的基因操纵和对cytoophidium完整性的分析.
- 流细胞计和显微镜以评估细胞周期阶段分布和细胞大小.
- 基因表达分析 (例如,定量PCR) 以确定受影响的细胞通路.
- 涉及候选基因过度表达的遗传救援实验.
主要成果:
- 在CTPS中发生的一种特定突变 (His359Ala) 导致了cytoophidia的分解.
- 降低CTPS蛋白水平或细胞的丧失导致了长时间的G2阶段和分裂酵母细胞大小的扩大.
- 损失CTPS细胞ophidia突变体表现出参与G2/M过渡和细胞生长的基因的表达减少,包括组织蛋白陪伴者slm9.
- 过度表达slm9部分挽救了G2阶段延长和细胞大小增加的表型,与CTPS细胞迷失相关.
结论:
- 在调节细胞循环进展和细胞大小控制中,CTPS细胞具有重要作用. Schizosaccharomyces pombe.
- 细胞的破坏会影响关键细胞周期和与生长相关的基因的表达.
- 基因组伴侣素slm9参与细胞对CTPS细胞质损失的反应,这表明细胞质和细胞周期期间的染色质调节之间存在联系.
关键词:
在CTP合成酶中,G2 阶段 G2 阶段这种植物是Schizosaccharomyces pombe.细胞循环中的细胞循环.细胞大小 细胞大小赛托奥菲迪乌姆 (cytoophidium) 是一个生物.这就是SLM9的意义.更多相关视频
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