工业用状真菌中的蛋白质分泌和相关压力
Reshma Jadhav1,2, Robert L Mach1, Astrid R Mach-Aigner3,4
1Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Str. 1a, A-1060, Vienna, Austria.
Applied microbiology and biotechnology
|January 11, 2024
概括
丝状真菌是工业酶生产的关键. 本综述探讨了它们的蛋白质分泌,未折叠蛋白质反应 (UPR) 和分泌应激 (RESS),突出了有线真菌的知识差距.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 纤维状真菌越来越多地用于商业酶和蛋白质生产,用于生物技术,食品和料应用.
- 在真菌中,蛋白质分泌可能受到来自高蛋白质合成的内质网膜 (ER) 压力所阻碍,导致蛋白质展开或错误折叠.
- 展开的蛋白质反应 (UPR) 是一种应对ER压力的细胞机制,并恢复蛋白质平衡.
研究的目的:
- 提供蛋白质分泌和相关应激反应在真菌的概述,专注于丝状真菌.
- 为了突出UPR通道跨真菌物种的保护性质.
- 强调需要进一步研究与工业相关的丝状真菌的分泌应激 (RESS).
主要方法:
- 审查现有的关于真菌蛋白质分泌和应激反应的文献.
- 在不同的真菌物种中对UPR通路的比较分析,包括Saccharomyces cerevisiae和丝状真菌.
- 检查在真菌中报告的分泌应激 (RESS) 的情况和条件.
主要成果:
- 在许多真菌中保留了UPR通路,尽管涉及的特定蛋白质可能有所不同.
- 在特定的压力条件下 (例如,与dithiothreitol,tunicamycin) 在Trichoderma reesei等一些丝状真菌中报告了分泌应激 (RESS).
- 在Saccharomyces cerevisiae中,RESS似乎不存在或不清楚表征.
结论:
- 丝状真菌对工业酶生产至关重要,因此需要更深入地了解它们的蛋白质分泌机制.
- 虽然UPR是一种保存反应,但RESS在工业重要线状真菌中理解较少,需要进一步研究.
- 未来的研究应该专注于阐明丝状真菌中RESS的机制,以优化蛋白质生产过程.
相关概念视频
Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Role of ER in the Secretory Pathway
5.4K
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
5.4K
Yeast Signaling
14.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.6K
Cytoskeletal Proteins in Bacteria
3.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.4K
Actin Filament Depolymerization
3.1K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
In F-actin, the ADF/cofilin proteins...
3.1K


