一个工程微生物联盟为Bacillus subtilis的素生产提供了前体
Si-Yu Wei1, Geng-Rong Gao1, Ming-Zhu Ding1
1Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 135 Yaguan Road, Jinnan District, Tianjin 300350, People's Republic of China.
Journal of natural products
|January 11, 2024
概括
这项研究将微生物改造为产生素,一种强大的抗真菌化合物. 在一个联盟中共同培养这些微生物显著提高了素的生产,显示了其作为食品防腐剂的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 代谢工程是代谢工程.
背景情况:
- 素是一种生物安全降解化合物,具有生物控制的潜力.
- 优化素生产对于其作为食品防腐剂和生物控制中的应用至关重要.
- 细菌细菌是众所周知的素生产者,但可以提高生产水平.
研究的目的:
- 为了设计Corynebacterium glutamicum用于高前体生产 (氨基酸).
- 开发一个三菌株的微生物联盟,以增强素生物合成.
- 为了评估基素对Rhizoctonia solani的抗真菌功效.
主要方法:
- 经过工程设计的Corynebacterium glutamicum可以过度产生三素,素,素和单素.
- 共同培养的工程C. glutamicum和Yarrowia lipolytica与Bacillus subtilis在一个三个菌株的联盟.
- 在5升生物反应器中优化素生产.
主要成果:
- 在财团中,素的产量达到了2100 mg·L-1.
- 在5L生物反应器中,素的产量进一步提升至3290mg·L-1.
- 芬吉辛在对抗Rhizoctonia solani.有显著的抗真菌活性.
结论:
- 使用微生物联盟和代谢工程开发了一种新的战略,用于增强素生产.
- 工程联盟显示出对工业规模素生产的前景.
- 素的抗真菌特性支持其作为食品防腐剂的潜在用途.
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