通过DNA框架传递的SiRNF8核酸有效地使结肠癌中的化疗敏感
Zhao Guo1, Haoyun Song1, Yingxia Tian2
1Department of Anatomy and Histology, Lanzhou University School of Basic Medical Sciences, Lanzhou, 730000, People's Republic of China.
International journal of nanomedicine
|January 11, 2024
概括
这项研究开发了一种新的DNA纳米载体 (si-DOX-Tet),用于为结肠癌提供化疗药物. 使用这种纳米载体抑制RNF8显著提高药物敏感性,并减少瘤生长.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 在瘤学瘤学.
背景情况:
- 化疗因副作用和降低药物敏感性而面临挑战.
- 基于纳米粒子的生物材料为克服这些局限性提供了一个有希望的解决方案.
研究的目的:
- 研究RNF8在结肠癌中的作用.
- 开发一种DNA四面体纳米载体,用于联合提供siRNF8和多克索鲁比 (DOX),用于组合治疗.
- 评估RNF8抑制在增强结肠癌细胞对DOX化疗敏感性的有效性.
主要方法:
- 生物信息学分析和结肠癌样本被用于评估RNF8表达.
- 构建了DNA四面体纳米载体 (si-Tet和si-DOX-Tet) 用于siRNF8和DOX传递.
- 为了评估治疗效果,进行了体外测试 (细胞增殖,DNA损伤,细胞亡,西斑,CCK-8).
主要成果:
- 在结肠癌中,RNF8表达升高.
- 成功合成了si-DOX-Tet纳米载体 (10.29-37.29nm),这些纳米载体被癌细胞内化并逃脱溶解体.
- si-RNF8治疗使RNF8沉默了~50%,增强了对化疗的敏感性并增加了DNA损伤.
- 通过降低ABCG2表达和DOX流量,si-DOX-Tet显著增加了亡 (1.6倍,而不是单独的DOX).
结论:
- 一种新的四面体DNA纳米载体 (si-DOX-Tet) 已成功地用于结肠癌联合治疗.
- 抑制RNF8增强了DOX化疗的敏感性,提供了一个新的治疗策略.
- 这种纳米载体系统为结合结肠癌治疗提供了一种有前途的方法.
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