研究超细气泡对细菌生长的影响
Mai Phuong Vu1, Nguyen Le Hanh Tran1, Thien Quang Lam1
1School of Biotechnology, International University, Vietnam National University Ho Chi Minh City 700000 Vietnam ntkhoi@hcmiu.edu.vn +84 28 3724 4271 +84 28 3724 4270.
RSC advances
|January 11, 2024
概括
超细气泡 (HUFB) 在大肠杆菌和黄金菌中显著增强了细菌的生长和繁殖. HUFBs促进细胞密度,但减少平均细菌大小,表明复杂的相互作用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 超细气泡 (UFB) 以其多样化的应用而闻名.
- UFB与微生物之间的相互作用具有重要的研究兴趣.
- UFBs的气核影响微生物生长调节.
研究的目的:
- 研究超细气泡 (UFB) 对细菌生长动态的影响.
- 为了比较空气UFB和UFB (HUFB) 对细菌模型的影响.
主要方法:
- 使用大肠杆菌和金黄色葡萄球菌作为模型生物.
- 在标准的Luria-Bertani (LB) 介质中培养的细菌,空气UFB注入的LB,和HUFB注入的LB.
- 通过超声波化生成的UFB,用SDS (50μM) 稳定,以确保影响微不足道.
主要成果:
- 补充HUFBs的培养物中细菌生长率最高.
- UFBs显示细胞增殖的明显增强和细胞密度的增加.
- 在HUFB介质中的细菌显著减少了平均形态大小 (p < 0.05).
结论:
- 超细气泡促进细菌生长,而UFB显示出更好的效果.
- HUFBs增强细胞增殖和密度,同时诱导细菌细胞大小的减少.
- 平均细菌形态大小和种群大小之间存在负相关性.
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