来自HarmoSter研究的报告:不同的LC-MS/MS和androstenedione,DHEAS和的方法相比较好;然而,统一校准是一种双刃剑
Flaminia Fanelli1, Mirko Peitzsch2, Stephen Bruce3
1Department of Medical and Surgical Sciences, Endocrinology Research Group, Center for Applied Biomedical Research, Alma Mater Studiorum University of Bologna, Bologna, Italy.
Clinical chemistry and laboratory medicine
|January 11, 2024
概括
这项研究发现,虽然液体染色学-并发质谱法 (LC-MS/MS) 对安德罗斯坦,脱水二硫酸 (DHEAS) 和的方法显示出很好的精度,但校准统一改进了对安德罗斯坦和DHEAS的协议,而不是的协议.
科学领域:
- 临床化学 临床化学
- 质谱测量质量谱测量
- 内分泌学 在内分泌学.
背景情况:
- 液体染色学-双重质谱法 (LC-MS/MS) 广泛用于循环测量.
- 在LC-MS/MS应用中的技术多样性导致了不同的结果,特别是对于,以及对二和脱水二硫酸盐 (DHEAS) 的有限数据.
研究的目的:
- 评估9个欧洲中心的androstenedione,DHEAS和的LC-MS/MS测量结果的一致性.
- 探索统一校准系统对这些雄激素测量的好处.
主要方法:
- 在78个患者样本和EQA材料中,使用实验室特定的LC-MS/MS程序测量了两次雄激素.
- 用内部和外部校准方法来评估结果.
- 评估了实验室内部和实验室间的表现.
主要成果:
- 实验室内变化系数 (CVs) 一般较低,表明高精度.
- 实验室间CVs为12.0%的androstenedione和7.2%的DHEAS与内部校准,提高到9.6%和4.9%,分别与外部校准.
- 校准系统统一显著改善了androstenedione和DHEAS的协议,但对测量没有显著的好处.
结论:
- LC-MS/MS方法显示了对雄激素的高实验室内精度.
- 对雄激素的实验室间协议很好,对安德罗二和DHEAS的校准统一提高了它.
- 测量的变化可能源于校准器的可换性和内部标准选择.
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