人类头皮的功能代谢:一种代谢利基,用于Staphylococcus epidermidis
Louis-Félix Nothias1,2, Robin Schmid1,2,3, Allison Garlet4
1Collaborative Mass Spectrometry Innovation Center, University of California San Diego, La Jolla, California, USA.
mSystems
|January 11, 2024
概括
我们开发了一种功能代谢学策略,以了解人类头皮中的代谢物功能. 这种方法整合了先进的数据分析工具和元基因组学,以确定Staphylococcus epidermidis的代谢利基.
科学领域:
- 系统生物学和与宿主相关的微生物群研究.
- 用于微生物组分析的代谢学和代谢基因组学集成.
背景情况:
- 从结构和丰度数据中解读代谢物功能仍然是一个挑战.
- 甲基因组学识别了微生物,但没有确定它们的活性作用;代谢组学揭示了环境化学,但往往有较低的注释率.
- 了解宿主-微生物相互作用需要将微生物存在与其代谢活动联系起来.
研究的目的:
- 开发和应用功能性代谢学策略,用于全面的代谢物注释.
- 在代谢学中解决"数据转化为知识"的挑战.
- 将代谢学与元基因组学整合起来,以阐明人类头皮上的微生物代谢.
主要方法:
- 将功能代谢学策略应用于从健康志愿者获得的人类头皮皮肤样.
- 使用液体染色学-并联质谱学 (LC-MS/MS) 与逆相 (C18) 和水性相互作用 (HILIC) 染色学,以及电喷射电离 (ESI) +/- 模式.
- 采用先进的数据分析工具:全球自然产品社交网络,SIRIUS和MicrobeMASST用于注释,MMvec用于整合代谢学和转基因组学数据.
主要成果:
- 实现了越来越全面的代谢物的结构和功能注释.
- 成功结合了代谢学和转基因组学数据.
- 获得了对人类皮肤上 *Staphylococcus epidermidis* 的特定代谢利基的见解.
结论:
- 开发的功能代谢学策略增强了代谢物注释和解释.
- 与元基因组学相结合,可以更深入地了解微生物在宿主环境中的作用.
- 这种方法成功地确定了 *Staphylococcus epidermidis* 的代谢利基,这是一个关键的皮肤共生物.
相关概念视频
Introduction to the Human Microbiota
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity, and disease...
The Skin Microbiota
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
Microbiome of the Eye
The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
Microbiota of the Large Intestine
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
Staphylococcal Skin Infections
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...


