在肯尼亚的一个动物收容所里流传的狗类Staphylococcaceae
Hatice Akarsu1,2, Anne M Liljander3, Anna Lacasta3
1Institute of Veterinary Bacteriology, University of Bern, Länggassstrasse, Bern, Switzerland.
Microbiology spectrum
|January 11, 2024
概括
这项研究调查了肯尼亚动物收容所的Staphylococcaceae细菌,发现了高抗生素耐药性和两个主要的Staphylococcus aureus克隆,可能从人类传播. 这为撒哈拉以南非洲的犬类抗菌药物治疗提供了关键的基线数据.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因组学就是基因组学.
- 一个健康,一个健康.
背景情况:
- 动物收容所,特别是在资源有限的地区,集中动物,增加传染病的风险,如呼吸道感染.
- 来自撒哈拉以南非洲的微生物学数据,特别是关于狗等伴侣动物的数据,非常稀缺.
- 了解庇护环境中的细菌种群及其耐药性概况对于动物健康和公共安全至关重要.
研究的目的:
- 从肯尼亚动物收容所的狗身上分离出来的Staphylococcaceae细菌的特征.
- 确定这些细菌的抗生素耐药性概况,遗传关系和主要克隆.
- 提供基线数据以指导该地区狗的抗微生物治疗策略.
主要方法:
- 2015年6月,从肯尼亚一个庇护所的167只狗身上收集了细菌样本.
- 通过标准种植和PacBio长读测序,分离并识别了92种Staphylococcaceae菌株.
- 进行了抗微生物敏感性测试 (最小抑制度) 和全基因组测序.
主要成果:
- 确定了九种经过验证的Staphylococcaceae物种,其中Staphylococcus aureus,S. pseudintermedius和Mammaliicoccus sciuri是主要的.
- 在92种菌株中,有86种菌株对至少一种已测试的抗微生物药物表现出耐药性.
- 在大约50%的狗中发现了两种主要的金黄色菌克隆 (ST15和ST1292),这表明潜在的人类起源和庇护所内传播.
- 新型的M. sciuri和S. pseudintermedius序列类型表明了撒哈拉以南的特定血统.
结论:
- 这项研究突出了肯尼亚一家狗收容所存在的抗生素耐药性葡萄球菌 (Staphylococcaceae) 的严重负担,包括潜在的动物感染性黄金色杆菌克隆.
- 这些发现强调了需要在撒哈拉以南非洲的动物收容所改善卫生和抗菌药物管理.
- 这项研究提供了重要的基因组和耐药性数据,对于该地区的兽医临床决策和公共卫生监测至关重要.
相关概念视频
01:29Staphylococcal Skin Infections
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
01:26Rocky Mountain Spotted Fever
Rocky Mountain Spotted Fever (RMSF) is a severe tick-borne illness caused by Rickettsia rickettsii, a Gram-negative, coccobacillary bacterium. This pathogen is an obligate intracellular parasite, requiring a host cell for replication. Transmission occurs through the bite of an infected tick. In the United States, the most important vectors are Dermacentor variabilis (American dog tick) and Dermacentor andersoni (Rocky Mountain wood tick), though other tick species may also serve as vectors.
01:25Clinical Significance of Antibiotic Resistance
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...