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模块MdCBF1/2-MdTST1/2调节糖的积累,以应对果中低温的情况
Baiyun Li1, Shengtao Qu1, Jiayi Kang1
1State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
The Plant journal : for cell and molecular biology
|January 11, 2024
概括
低温通过增加可溶糖增加果的甜味. 这由MdCBF1/2-MdTST1/2基因通路控制,这对水果质量至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 溶性糖含量显著影响水果的风味和甜度.
- 温度,特别是白天的温度范围,会影响肉质水果中的糖累积.
- 将温度与水果糖含量联系在一起的精确分子机制尚未完全理解.
研究的目的:
- 调查果中低温诱导的可溶糖积累的分子基础.
- 为了确定关键的基因和转录因子参与这个过程.
- 为了阐明控制糖累积的调节途径,以应对温度.
主要方法:
- 对转化酶可访问的染色体 (ATAC) 测序进行测试.
- 基因表达分析.
- 对cis作用元素和转录因子结合的分析.
- 在果果和水果中过度表达和沉默基因.
主要成果:
- 低温处理上调调节了Tonoplast糖载体 (MdTST1/2) 基因,促进了糖的积累.
- 两个C-重复结合因子 (MdCBF1和MdCBF2) 被确定为MdTST1/2.2的上游转录因子.
- MdCBF1/2直接与MdTST1/2促进体结合,激活它们的表达并增加葡萄糖,果糖和糖水平.
- MdCBF1/2-MdTST1/2模块对于果中低温中介糖的积累至关重要.
结论:
- 调节模块MdCBF1/2-MdTST1/2在低温下对果糖的积累起着至关重要的作用.
- 这一途径提供了对环境温度如何影响水果质量的机制性见解.
- 这些发现提供了通过基因操纵改善水果甜味的潜在策略.
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